Anti-b7-h3 antibodies and antibody drug conjugates

Abstract

The invention relates to B7 homology 3 protein (B7-H3) antibodies and antibody drug conjugates (ADCs), including compositions and methods of using said antibodies and ADCs.

1 . An isolated anti-hB7H3 antibody, wherein the antibody comprises
 a heavy chain variable region comprising
 a CDR3 having an amino acid sequence selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 35, and SEQ ID NO: 27. 
   and a light chain variable region comprising
 a CDR3 having an amino acid sequence selected from the group consisting of of SEQ ID NO: 15, SEQ ID NO: 39, and SEQ ID NO: 31; 
 a CDR2 having an amino acid sequence selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 38, and SEQ ID NO: 30; and 
 a CDR1 having an amino acid sequence selected from the group consisting of SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 37, SEQ ID NO: 14, and SEQ ID NO: 29. 
   
     
     
         2 - 12 . (canceled) 
     
     
         13 . The antibody according to  claim 1 , wherein the antibody further comprises a human acceptor framework and said human acceptor framework comprises an amino acid sequence selected from the group consisting of SEQ ID 155, 156, 164, 165, 166, and 167. 
     
     
         14 - 22 . (canceled) 
     
     
         23 . The antibody according to  claim 1 , wherein the antibody further comprises a human acceptor framework and said human acceptor framework comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 155 to 158. 
     
     
         24 - 31 . (canceled) 
     
     
         32 . The anti-hB7-H3 antibody according to  claim 1 , wherein the antibody comprises a heavy chain variable domain comprising an amino acid sequence set forth in SEQ ID NO: 139 and a light chain variable domain comprising an amino acid sequence set forth in SEQ ID NO: 135. 
     
     
         33 . (canceled) 
     
     
         34 . The anti-hB7-H3 antibody according to  claim 1 , wherein the antibody comprises a heavy chain variable domain comprising an amino acid sequence set forth in SEQ ID NO: 139 and a light chain variable domain comprising an amino acid sequence set forth in SEQ ID NO: 137. 
     
     
         35 . (canceled) 
     
     
         36 . The anti-hB7-H3 antibody according to  claim 1 , wherein the antibody comprises a heavy chain variable domain comprising an amino acid sequence set forth in SEQ ID NO: 147 and a light chain variable domain comprising an amino acid sequence set forth in SEQ ID NO: 144. 
     
     
         37 - 129 . (canceled) 
     
     
         130 . The isolated antibody according to  claim 1 , wherein the antibody, or antigen binding portion thereof, comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 168 and a light chain comprising the amino acid sequence of SEQ ID NO: 169. 
     
     
         131 . The isolated antibody, according to  claim 1 , wherein the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 170 and a light chain comprising the amino acid sequence of SEQ ID NO: 171. 
     
     
         132 . The isolated antibody according to  claim 1 , wherein the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 172 and a light chain comprising the amino acid sequence of SEQ ID NO: 173. 
     
     
         133 - 137 . (canceled)