USRE44401EExpiredUtility

Thyroid hormone analysis by mass spectrometry

Individually held — no corporate assignee on recordPriority: Apr 14, 2003Filed: Nov 17, 2011Granted: Jul 30, 2013

Est. expiryApr 14, 2023(expired)· nominal 20-yr term from priority

Inventors:Steven J. Soldin

G01N 33/78Y10T436/24G01N 33/6848

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References

Claims

Abstract

Methods, systems and kits for the simultaneous or sequential analysis of one or more hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods include ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of thyroid hormones are disclosed including ionization of the thyroid hormones in the negative mode using an electrospray source.

Claims

exact text as granted — not AI-modified

What is claimed is:

1. A method for mass spectrometric analysis of a 100 μL sample comprising one or more thyroid hormones, the method consisting essentially of the steps of: (a) providing a sample comprising one or more thyroid hormones, wherein the sample is approximately 100 μL; (b) deproteinating the sample; (c) separating the one or more thyroid hormones from the sample; and (d) analyzing the one or more thyroid hormones using a mass spectrometer.

2. The method according to claim 1 wherein the one or more thyroid hormones are selected from the group consisting of T3 and T4.

3. The method according to claim 1 wherein the sample comprising one or more thyroid hormones is obtained from a biological sample selected from the group consisting of blood, plasma, serum, urine and saliva.

4. The method of claim 3 wherein the biological sample is blood.

5. The method of claim 3 wherein the biological sample is plasma.

6. The method of claim 3 wherein the biological sample is serum.

7. The method of claim 3 wherein the biological sample is urine.

8. The method of claim 3 wherein the biological sample is saliva.

9. The method according to claim 1 wherein said step of deproteinating the sample comprises:
(a) adding acetonitrile, containing internal standards;
(b) vortexing the sample; and
(c) subjecting the sample to centrifugation.

10. The method according to claim 1 wherein said step of deproteinating the sample comprises subjecting the sample to precipitation with an agent containing internal standards, said agent selected from the group consisting of methanol, ethanol and salt.

11. The method according to claim 1 wherein said step of separating the one or more thyroid hormones from the sample comprises introducing the sample to a liquid chromatography apparatus comprising a column and subsequently eluting the thyroid hormones from the column.

12. The method according to claim 11 wherein said step of separating the one or more thyroid hormones from the sample comprises the use of a C-18 column.

13. The method according to claim 1 wherein said step of separating the one or more thyroid hormones from the sample comprises the use of a combined liquid chromatography spectrometry apparatus.

14. The method according to claim 13 wherein the one or more thyroid hormones are introduced into a mass spectrometer directly after being separated from the sample by way of an on-line extraction and use of a built-in switch valve.

15. The method according to claim 1 wherein the mass spectrometer is a liquid chromatography-tandem-mass spectrometer.

16. The method according to claim 15 wherein the liquid chromatography-tandem mass spectrometer is equipped with an electrospray ionization source.

17. The method according to claim 1 wherein said step of analyzing the one or more thyroid hormones using a mass spectrometer comprises an ionization technique selected from the group consisting of photoionization, electrospray ionization, atmospheric pressure chemical ionization, and electron capture ionization.

18. The method according to claim 17 wherein said ionization technique is electrospray ionization.

19. The method according to claim 17 wherein said ionization is performed in positive mode.

20. The method according to claim 17 wherein said ionization is performed in negative mode.

21. The method according to claim 1 wherein said step of analyzing the one or more thyroid hormones using a mass spectrometer comprises multiple reaction monitoring.

22. The method according to claim 1 wherein said step of analyzing the one or more thyroid hormones using a mass spectrometer comprises selected ion monitoring.

23. The method according to claim 1 wherein the sample comprises a plurality of thyroid hormones and they are analyzed simultaneously.

24. The method according to claim 1 wherein the sample comprises a plurality of thyroid hormones and they are analyzed sequentially.

25. The method according to claim 1 wherein the sample is analyzed by isotope dilution tandem mass spectrometry.

26. A method of instructing an analysis of a sample comprising one or more thyroid hormones, the method comprising providing instructions to prepare the sample according to steps (b) and (c) of claim 1 and analyze the one or more thyroid hormones from the sample according to step (d) of claim 1 .

27. A system for the mass spectrometric analysis of a sample comprising one or more thyroid hormones, and wherein the sample is approximately at least about 100 μL, said system comprising:
(a) reagents for deproteinating the sample, including internal standards;
(b) reagents for analyzing the sample comprising one or more thyroid hormones using a mass spectrometer; and
(c) a mass spectrometer.

28. The system according to claim 27 wherein the mass spectrometer is a liquid chromatography-tandem mass spectrometer.

29. A kit for use in mass spectrometric analysis of a sample comprising one or more thyroid hormones, and wherein the sample is approximately at least about 100 μL, said kit comprising:
(a) reagents for deproteinating the sample comprising one or more thyroid hormones, including internal standards;
(b) reagents for separating the one or more thyroid hormones from the sample comprising one or more thyroid hormones;
(c) reagents for analyzing the one or more thyroid hormones using a mass spectrometer;
(d) a solution of one or more thyroid hormones; and
(e) instructions for analyzing the one or more thyroid hormones using a mass spectrometer,

wherein the analysis does not involve an extraction step comprising evaporation and redissolving the extracted thyroid hormone, and the analysis is done in less than an hour.

30. The kit according to claim 29 further comprising:
(a) mobile phase solutions;
(b) a chromatography column; and
(c) a quality control specimen.

31. Use of A method for using a mass spectrometer for sequentially or simultaneously analyzing a 100 uL sample comprising or suspected of comprising a plurality of thyroid hormones, wherein the use method consists of the steps of:
(a) providing a sample comprising or suspected of comprising a plurality of thyroid hormone, wherein the sample is approximately at least about 100 μL;
(b) deproteinating the sample;
(c) separating the plurality of thyroid hormones from the sample; and
(d) analyzing the plurality of thyroid hormones using a mass spectrometer.

32. The use method according to claim 31 wherein the mass spectrometer is a liquid chromatography-tandem mass spectrometer.

33. The method of any one of claims 1 wherein the mass spectrometer is an API 2000™ mass spectrometer.

34. The method of any one of claims 1 wherein the mass spectrometer is an API 3000™ mass spectrometer.

35. A method for mass spectrometric analysis of a sample comprising one or more thyroid hormones, the method comprising the steps of:
(a) providing a sample comprising one or more thyroid hormones, wherein the sample is at least about 100 μL; (b) deproteinating the sample; (c) separating the one or more thyroid hormones from the sample; and (d) analyzing the one or more thyroid hormones using a mass spectrometer.

36. The method according to claim 35 wherein the one or more thyroid hormones are selected from the group consisting of T3 and T4.

37. The method according to claim 35 wherein the sample comprising one or more thyroid hormones is obtained from a biological sample selected from the group consisting of blood, plasma, serum, urine and saliva.

38. The method of claim 37 wherein the biological sample is blood.

39. The method of claim 37 wherein the biological sample is plasma.

40. The method of claim 37 wherein the biological sample is serum.

41. The method of claim 37 wherein the biological sample is urine.

42. The method of claim 37 wherein the biological sample is saliva.

43. The method according to claim 35 wherein said step of deproteinating the sample comprises:
(a) adding acetonitrile, containing internal standards; (b) vortexing the sample; and (c) subjecting the sample to centrifugation.

44. The method according to claim 35 wherein said step of deproteinating the sample comprises subjecting the sample to precipitation with an agent containing internal standards, said agent selected from the group consisting of methanol, ethanol and salt.

45. The method according to claim 35 wherein said step of separating the one or more thyroid hormones from the sample comprises introducing the sample to a liquid chromatography apparatus comprising a column and subsequently eluting the thyroid hormones from the column.

46. The method according to claim 45 wherein said step of separating the one or more thyroid hormones from the sample comprises the use of a C-18 column.

47. The method according to claim 35 wherein said step of separating the one or more thyroid hormones from the sample comprises the use of a combined liquid chromatography spectrometry apparatus.

48. The method according to claim 47 wherein the one or more thyroid hormones are introduced into a mass spectrometer directly after being separated from the sample by way of an on-line extraction and use of a built-in switch valve.

49. The method according to claim 35 wherein the mass spectrometer is a liquid chromatography-tandem-mass spectrometer.

50. The method according to claim 49 wherein the liquid chromatography-tandem mass spectrometer is equipped with an electrospray ionization source.

51. The method according to claim 35 wherein said step of analyzing the one or more thyroid hormones using a mass spectrometer comprises an ionization technique selected from the group consisting of photoionization, electrospray ionization, atmospheric pressure chemical ionization, and electron capture ionization.

52. The method according to claim 51 wherein said ionization technique is electrospray ionization.

53. The method according to claim 51 wherein said ionization is performed in positive mode.

54. The method according to claim 51 wherein said ionization is performed in negative mode.

55. The method according to claim 35 wherein said step of analyzing the one or more thyroid hormones using a mass spectrometer comprises multiple reaction monitoring.

56. The method according to claim 35 wherein said step of analyzing the one or more thyroid hormones using a mass spectrometer comprises selected ion monitoring.

57. The method according to claim 35 wherein the sample comprises a plurality of thyroid hormones and they are analyzed simultaneously.

58. The method according to claim 35 wherein the sample comprises a plurality of thyroid hormones and they are analyzed sequentially.

59. The method according to claim 35 wherein the sample is analyzed by isotope dilution tandem mass spectrometry.

60. A method of instructing an analysis of a sample comprising one or more thyroid hormones, the method comprising providing instructions to prepare the sample according to steps (b) and (c) of claim 35 and analyze the one or more thyroid hormones from the sample according to step (d) of claim 35.

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