Canine erythropoietin gene and recombinant protein

Abstract

One aspect of the present invention is an isolated nucleic acid molecule encoding canine erythropoietin. The present invention also relates to an isolated canine erythropoietin protein or polypeptide. Another aspect of the present invention is a method for providing erythropoietin therapy to a dog or a cat including administering recombinant canine erythropoietin to a dog or a cat in need of erythropoietin therapy is an amount sufficient to increase production or reticulocytes and red blood cells in the dog or cat.

1. An isolated nucleic acid molecule encoding a canine erythropoietin, wherein the canine erythropoietin has an  the amino acid sequence of SEQ ID NO:3. 
     
     
       2. The nucleic acid molecule of  claim 1 , wherein the nucleic acid molecule comprises bases 361-4491 of the nucleotide sequence of SEQ ID NO:1. 
     
     
       3. The nucleic acid molecule of  claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:2. 
     
     
       4. An expression vector comprising the nucleic acid molecule of  claim 1 , wherein the nucleic acid is foreign to the expression vector. 
     
     
       5. An expression vector according to  claim 4 , wherein the expression vector is pLEN. 
     
     
       6. An expression vector according to  claim 6 , wherein the nucleic acid molecule is inserted into the expression vector in proper sense orientation and correct reading frame. 
     
     
       7. A host cell comprising the expression vector of  claim 4 . 
     
     
       8. The host cell according to  claim 7 , wherein the host-cell is a mammalian-cell. 
     
     
       9. The host cell according to  claim 8 , wherein the mammalian host cell is a Chinese hamster ovary cell. 
     
     
       10. A host cell comprising the isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid molecule is foreign to the host cell. 
     
     
       11. An isolated canine erythropoietin protein or polypeptided  polypeptide having an  the amino acid sequence of SEQ ID NO:3. 
     
     
       12. The protein or polypeptide according to  claim 11 , wherein the protein or polypeptide is encoded by a nucleic acid molecule comprising bases 361-4491 of the nucleotide of SEQ ID NO:1. 
     
     
       13. The protein or polypeptide according to  claim 11 , wherein the protein or polypeptide is encoded by a nucleic acid molecule having an  the nucleotide sequence of SEQ ID NO:2. 
     
     
       14. A pharmaceutical composition comprising:
 a protein or polypeptide according to  claim 11  and  
 a pharmaceutically acceptable diluent, adjuvant, or carrier.  
 
     
     
       15. A method for providing erythropoietin therapy to a dog comprising:
 administering an effective amount of the pharmaceutical composition of  claim 14  to the dog.  
 
     
     
       16. A method for providing erythropoietin therapy to a cat comprising:
 administering an effective amount of the pharmaceutical composition of  claim 14  to the cat.