Galactopyranosides and their use

Abstract

The present invention relates to simplified synthesis, new carbohydrate-based products and practical use of different carbohydrate-based products. Examples of these are (Galα1-3Gal), GlcNAcβ1-3Gal, α- or β-glycosides thereof, Galα1-3Gal- containing tri-, or higher oligosaccharides, α- or β-glycosides thereof, GlcNAcβ1-3Gal containing tri-, tetra-, or higher oligosaccharides, and derivatives and/or α- or β-glycosides thereof, Galα1-3GalGlcNAcβ1-3Gal, α- or β-glycosides thereof, Galα1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc, or other higher oligosaccharides containing the Galα1-3Gal-structure, α- or β-glycosides thereof, modified carbohydrates, di-, tri-, oligo-, or polyfunctional products containing carbohydrate structures, and the use of the products for synthesis, affinity purification, diagnostic applications and therapy.

1. The saccharide compound Galα1-3Galβ1-4GlcNAcβ-OEtPhNH— CO(CH 2 ) 5 NH—CH 2 —CH(OH)—CH 2 —O-Sepharose® 4FF  agarose. 
     
     
       2. A method of making the saccharide compound of  claim 1  comprising:
 reacting carbohydrate —OCH 2 CH 2 PhNH 2  with NHS-activated Sepharose 4FF  agarose to obtain the desired product.  
 
     
     
       3. A method of making the saccharide compound of  claim 1  comprising:
 reacting Galα1-3Galβ1-4GlcNAcβ-OPHNH 2  with NHS-activated Sepharose-4FF  agarose.  
 
     
     
       4. A method for the treatment of a patient in connection with and after xenotransplantation of an organ in said patient comprising contacting plasma of the patient with the saccharide compound of  claim 1  for the elimination of antibodies against said saccharide compound from the plasma. 
     
     
       5. A method for the removal of at least one of cells, proteins and enzymes from a plasma sample taken from a patient in need thereof comprising contacting said plasma sample with the saccharide compound of  claim 1  and thereby isolating or removing said at least one of said cells, proteins and enzymes. 
     
     
       6. The method according to  claim 5  wherein glycosyltransferases, lectins or antibodies are isolated. 
     
     
       7. A method for the diagnosis for the presence of at least one member of the group consisting of bacteria, cells, enzymes, anti-bodies and carbohydrate bound proteins having specificity towards a saccharide in a body fluid from a patient in need of said diagnosis comprising contacting said fluid with the saccharide compound of  claim 1 . 
     
     
       8. The method according to  claim 7  wherein said saccharide is present in a biosensor or in an ELISA.