DNA sequence coding for protein C

Abstract

Genomic and cDNA sequences coding for a protein having substantially the same biological activity as human protein C are disclosed. Recombinant plasmids and bacteriophage transfer vectors incorporating these sequences are also disclosed.

1. An isolated human DNA sequence which codes for a protein having substantially the same biological activity as human protein C. 
     
     
       2. An isolated DNA sequence comprising the sequence of  FIG. 2 , from bp 1 to bp 8972, which sequence codes for human protein C. 
     
     
       3. A bacterial plasmid for bacteriophage transfer vector comprising a cDNA sequence comprising the human protein C gene cDNA sequence. 
     
     
       4. An isolated human DNA which codes for human protein C, wherein said DNA comprises a sequence which codes for amino acids  1  to  419  as shown in  FIG. 3.   
     
     
       5. The isolated human DNA of  claim 4 , wherein said sequence codes for the amino acid sequence of  FIG. 3 , starting with methionine, number − 42 , and ending with proline, number  419 . 
     
     
       6. The isolated human DNA of  claim 4 , wherein said DNA comprises nucleotides  127 - 1383  of  FIG. 3.   
     
     
       7. The isolated human DNA of  claim 6 , wherein said DNA comprises nucleotides  1 - 1383  of  FIG. 3.   
     
     
       8. The isolated human DNA of  claim 4 , wherein said DNA comprises nucleotides  1390 - 1500 ,  2963 - 2987 ,  3080 - 3217 ,  3320 - 3453 ,  6123 - 6265 ,  7139 - 7256 , and  8386 - 8972  as shown in  FIG. 2.   
     
     
       9. The isolated human DNA of  claim 8 , wherein said DNA comprises nucleotides  1 - 70 ,  1334 - 1500 ,  2963 - 2987 ,  3080 - 3217 ,  3320 - 3453 ,  6123 - 6265 ,  7139 - 7256 , and  8386 - 8972  as shown in  FIG. 2.   
     
     
       10. An isolated human DNA which codes for human protein C, wherein said human protein C comprises a light chain as shown in  FIG. 3  from amino acid number  1  to amino acid number  155 , and a heavy chain as shown in  FIG. 3  from amino acid number  158  to amino acid number  419 . 
     
     
       11. An isolated human DNA which codes for human protein C, wherein said DNA consists of nucleotides  1 - 1383  of  FIG. 3.   
     
     
       12. An isolated human DNA which codes for human protein C, wherein said DNA consists of nucleotides  1 - 70 ,  1334 - 1500 ,  2963 - 2987 ,  3080 - 3217 ,  3320 - 3453 ,  6123 - 6265 ,  7139 - 7256 , and  8386 - 8972  as shown in  FIG. 2.