Anti-aids immunotoxins

Abstract

The present invention provides a novel anti-AIDS immunotoxin. The immunotoxin comprises a toxin chemically conjugated to a monoclonal antibody directed against viral reverse transcriptase. Also provided are various methods of using this novel immunotoxin including methods of treating various diseases.

What is claimed is: 
     
       1. A composition of matter, comprising an immunotoxin, said immunotoxin comprising a toxin chemically conjugated to a monoclonal antibody directed against an HIV viral reverse transcriptase. 
     
     
       2. The composition of claim 1, wherein said toxin is selected from the group consisting of pokeweed antiviral protein, gelonin, ricin, abrin, modeccin, dodecandrin, saporin, volkensin and vicumin. 
     
     
       3. The composition of claim 1, wherein said immunotoxin is chemically cross-linked using a cross-linking agent selected from the group consisting of m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), N-succinimidyl-3-(2-pyridyldithio-)-propionate (SPDP), alpha-iminothiolane hydrochloride, methyl 3-mercaptopropionimidate, Succinimidyl 4-(N maleimidomethyl) cyclohexane-1-carboxylate (SMCC), 4-succinimidyloxycarbonyl-alpha-methyl-alpha-(2-pyridydithio)-toluene (SMPT), N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) and sulfosuccinimidyl 4-(p-maleimidophenyl)butyrate (SMPB). .Iadd. 
     
     
       4.  A recombinantly produced immunotoxin, said immunotoxin comprising a toxin linked to a monoclonal antibody directed against an HIV viral reverse transcriptase..Iaddend..Iadd. 
     
     
       5.  A fusion protein, said protein comprising a toxin protein recombinantly fused to a monoclonal antibody directed against an HIV viral reverse transcriptase..Iaddend..Iadd.6. A fusion protein, said protein comprising gelonin protein recombinantly fused to a monoclonal antibody directed against an HIV viral reverse transcriptase..Iaddend.