Biological assembly

Abstract

A biological assembly for effecting the examination of a sample in a liquid medium under magnification. The assembly includes a transparent slide with a sample chamber and an immediately adjacent overflow chamber formed on one main surface by a thin film. The sample chamber is receptive of a sample in a liquid medium. transparent slide, having a pattern for aligning it with the sample chamber, is disposed over the sample chamber. The transparent slide includes a grid etched into the lower surface thereof to enable the counting cells under magnification.

What is claimed is: 
     
       1. A biological assembly for effecting the examination of a sample in a liquid medium under magnification, comprising: a transparent substrate having a first main surface;   means forming at least one sample chamber and an immediately adjacent overflow chamber on the first main surface and receptive of a sample in a liquid medium, said means comprising a thin film having for each sample chamber and overflow chamber, means defining a first opening therein corresponding to the sample chamber and means defining a second opening surrounding the first opening and corresponding to the overflow chamber, wherein the first and second openings form a separating wall of thin film therebetween; and   a transparent cover having first main surface superposable on the thin film to cover the at least one sample chamber and overflow chamber when a sample is received therein, wherein the cover has a second main surface opposite the first main surface and having a thin film pattern thereon demarcating an area for aligning the area of the cover with the at least one sampling chamber.   
     
     
       2. The biological assembly according to claim 1, wherein the first opening is circular and the second opening is toroidal. 
     
     
       3. The biological assembly according to claim 1, wherein the first opening is circular and the second opening is rectangular. 
     
     
       4. The biological assembly according to claim 1, wherein the thin film has a height of from 10 to 50 μm. 
     
     
       5. The biological assembly according to claim 1, wherein the separating wall has a width of 200 to 800 μm. 
     
     
       6. The biological assembly according to claim 1, wherein said at least one sample chamber has a volume of 2 to 15 μl. 
     
     
       7. The biological assembly according to claim 1, wherein the thin film pattern is identical in configuration to said separating wail. 
     
     
       8. The biological assembly according to claim 1, wherein the cover has a grid pattern in the first main surface within the area demarcated by said thin film pattern. 
     
     
       9. The biological assembly according to claim 1, wherein the thin film comprises at least one of a photoresist, an epoxy and a hydrophobic ink. 
     
     
       10. A biological assembly for effecting examination of a sample in a liquid medium under magnification, comprising: a transparent substrate having a first main surface;   means forming at least one sample chamber on the first main surface and receptive of a sample in a liquid medium, said means comprising a thin film having for each sample chamber, means defining an opening therein corresponding to the sample chamber and means defining a open area surrounding the opening, wherein the opening and open area form a separating wall of thin film therebetween; and   a transparent cover having fast main surface superposable on the thin film to cover the at least one sample chamber when a sample is received therein, wherein the cover has a second main surface opposite the first main surface and having a thin film pattern thereon demarcating an area for aligning the area of the cover with the at least one sampling chamber.   
     
     
       11. The biological assembly according to claim 10, wherein the opening is circular. 
     
     
       12. The biological assembly according to claim 10, wherein the thin film has a height of from 10 to 50 μm. 
     
     
       13. The biological assembly according to claim 10, wherein the separating wall has a width of 200 to 800 μm. 
     
     
       14. The biological assembly according to claim 10, wherein said at least one sample chamber has a volume of 2 to 15 μl. 
     
     
       15. The biological assembly according to claim 10, wherein the thin film pattern is identical in configuration to said separating wail. 
     
     
       16. The biological assembly according to claim 10, wherein the cover has a grid pattern in the first main surface within the area demarcated by said thin film pattern. 
     
     
       17. The biological assembly according to claim 10, wherein the thin film comprises at least one of a photoresist, an epoxy and a hydrophobic ink. .Iadd. 
     
     
       18.  A biological assembly for effecting examination of a sample in a liquid medium under magnification, comprising: a transparent substrate having a surface for receiving the sample;   a transparent cover having a first main surface superposed on the surface of the substrate during use and comprising a grid pattern and spacers disposed outwardly of the grid pattern for spacing the first main surface apart from the substrate surface by a distance when the cover is superposed on the substrate and a second main surface opposite the first main surface and having a first thin film pattern thereon demarcating an area including the grid pattern for aligning the grid pattern over the sample when the cover is superposed on the substrate. .Iaddend..Iadd.19. The biological assembly according to claim 18, wherein the first thin film pattern is a ring surrounding the grid pattern. .Iaddend..Iadd.20. The biological assembly according to claim 18, wherein the spacers comprise a second thin film pattern on the first main surface. .Iaddend..Iadd.21. The biological assembly according to claim 20, wherein the second thin film pattern comprises circles in corners of the cover. .Iaddend..Iadd.22. The biological assembly according to claim 18, wherein the spacers space the first main surface from the substrate surface by 10 microns. .Iaddend..Iadd.23. The biological assembly according to claim 18, wherein the spacers space the first main surface from the substrate surface by 20 microns. .Iaddend..Iadd.24. The biological assembly according to claim 18, wherein the spacers space the first main surface from the substrate surface by 50 microns. .Iaddend..Iadd.25. A biological assembly for effecting examination of a sample in a liquid medium under magnification comprising:   a transparent slide having a completely planar surface for receiving the sample;   a transparent slide cover having a first main surface superposed on the surface of the slide during use and comprising a grid pattern, a thin film pattern demarcating an area including the grid pattern for aligning the grid pattern over the sample when the slide cover is superposed on the slide and a plurality of discrete spacers disposed outwardly of and separated from the thin film pattern without enclosing same for spacing the first main surface apart from the slide surface by a distance when the slide cover is superposed on the slide. .Iaddend..Iadd.26. The biological assembly according to claim 25, wherein the thin film pattern is a ring surrounding the grid pattern. .Iaddend..Iadd.27. The biological assembly according to claim 25, wherein the spacers space the first main surface from the slide surface by 10 microns. .Iaddend..Iadd.28. The biological assembly according to claim 25, wherein the spacers space the first main surface from the slide surface by 20 microns. .Iaddend..Iadd.29. The biological assembly according to claim 25, wherein the spacers space the first main surface from the slide surface by 50 microns. .Iaddend.