Infectious bronchitis vaccine for poultry

Abstract

Infectious bronchitis (IB) vaccines for poultry derived from at least one novel virus strain of novel infectious bronchitis serotypes, selected from the group consisting of culture Nos. CNCTC A 07/80, CNCTC A 08/80, CNCTC A 09/80, CNCTC A 010/80, CNCTC A 011/80, CNCTC A 013/80, CNCTC A 014/80, CNCTC A 015/80 and CNCTC A 016/80 deposited at the Czechoslovak National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague, Czechoslovakia and the novel viruses per se, combined virus vaccaines and a novel method of protecting poultry from infectious bronchitis.

We claim: 
     
       1. A combined infectious bronchitis vaccine for poultry comprising at least one first virus fluid derived from a serotype virus strain of infectious bronchitis viruses selected from the group consisting of culture Nos. CNCTC A 07/80, CNCTC A 08/80, CNCTC A 09/80, CNCTC A 010/80, CNCTC A 011/80, CNCTC A 013/80, CNCTC A 014/80, CNCTC A 015/80 and CNCTC A 016/80 deposited at the Czechoslovak National Collection of Type Cultures with the Institute of Hygiene and Epidemiology in Prague, Czechoslovia and a second virus fluid selected from the group consisting of IBV H 120 and IBV H 52 of Massachusetts type. 
     
     
       2. A combined vaccine of claim 1 wherein the first virus strain is No. 07/80. 
     
     
       3. A combined vaccine of claim 1 wherein the first virus strain is No. 014/80. 
     
     
       4. A combined vaccine of claim 1 wherein the first virus strain is No. 015/80. 
     
     
       5. A combined vaccine of claim 1 wherein the first virus fluid is derived from infectious bronchitis virus strain No. 07/80. 
     
     
       6. A conbined vaccine of claim 1 wherein the second virus fluid is derived from the IBV H 120 of the Massachusetts type. 
     
     
       7. A live infectious bronchitis vaccine of claim 5 having a total virus content of ≧2×10 4 .0 EID 50  per dose of each of the virus strains. 
     
     
       8. A process for the preparation of a combined virus vaccine of claim 1 comprising cultivating at least one infectious bronchitis virus selected from the group consisting of culture Nos. CNCTC A 07/80, CNCTC A 08/80, CNCTC A 09/80, CNCTC A 010/80, CNCTC A 011/80, CNCTC A 013/80, CNCTC A 014/80, CNCTC A 015/80 and CNCTC A 016/80 deposited with the Czechoslovak National Collection of Type Cultures with the Institute of Hygiene and Epidemiology in Prague, Czechoslovia, collecting and purifying the cultivated virus material, optionally adding thereto stabilizers and/or antibiotics, cultivating an IBV H.120 or IBV H.52 virus, recovering and purifying the virus material, optionally adding thereto stabilizers and/or antibiotics, mixing the two virus materials together and subjecting the mixture to lyophilizating. 
     
     
       9. The process of claim 8 wherein the combined vaccine has a virus dose of ≧2×10 4 .0 EID 50  per dose of each of the virus components. 
     
     
       10. The process of claim 9 wherein the dose is ≧10 4 .0 EID 50 . .Iadd. 
     
     
       11.  A method of preventing infectious bronchitis in poultry comprising administering to poultry an amount of a combined vaccine of claim 1 sufficient to prevent infectious bronchitis..Iaddend. .Iadd.12. The method of claim 11 wherein the vaccine is derived from infectious bronchitis virus No. CNCTC A 07/80 of the Czechoslovakia National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague..Iaddend. .Iadd.13. The method of claim 11 wherein the vaccine is derived from infectious bronchitis virus No. CNCTC A 014/80 of the Czechoslovakia National Collection of Type Cultures of the Institute of Hygiene and 
     
     
        Epidemiology in Prague..Iaddend. .Iadd.14.  The method of claim 11 wherein the vaccine is derived from infectious bronchitis virus No. CNCTC A 015/80 of the Czechoslovakia National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague..Iaddend. .Iadd.15. The method of claim 11 wherein the second virus fluid is derived from IBV H 52 of the Massachusetts type..Iaddend. .Iadd.16. The method of claim 15 wherein the first virus fluid is derived from infectious bronchitis vaccine No. 07/80..Iaddend. .Iadd.17. The method of claim 11 wherein the second virus fluid is derived from IBV H 120 of the Massachusetts type..Iaddend.