Recovery of cellular protein products from microbiological synthesis masses

Abstract

Cellular protein products are recovered from microbiologically produced masses by incorporation therewith of a protein material suitable as a food which acts as a flocculant.

I claim: .[.1. In the process of recovering microbiologically produced cellular protein products useful as animal food from the medium in which they were produced by adjusting the pH to a level which will permit flocculation, adding a sufficient amount of a flocculating agent to cause flocculation, followed by physical separation, the improvement comprising utilizing as the flocculating agent a proteinaceous material capable of flocculating the cellular protein products..]. .[.2. The process according to claim 1 wherein the proteinaceous material is at least one of casein, gluten, skim milk powder, soy bean protein, gelatin, or zein..]. .[.3. The process according to claim 2 wherein the amount and the type of proteinaceous flocculating agent used are such as to obtain a recovered product containing a balanced mixture of amino acids..]. .[.4. The process according to claim 2 wherein the amount of proteinaceous flocculant employed in the recovery process is in the approximate range of from about 0.2 to about 25 weight percent of the total weight of the cells and growth medium..]. .[.5. The process according to claim 1 wherein the cells and the growth medium are at ambient temperature during the recovery process..]. .[.6. The process according to claim 5 wherein the pH of the cells and growth medium to which the flocculant is added is in the range of about 4to about 6..]. .[.7. The process according to claim 6 wherein the proteinaceous material is casein..]. .[.8. The process according to 
     
        claim 6 wherein the proteinaceous material is skim milk powder..]. .[.9. The method of recovering microbiologically produced cells at ambient temperature from the medium in which they were produced comprising adding to the medium a proteinaceous material capable of flocculating the cells in an amount sufficient to cause flocculation, said amount being at least about 0.2 weight percent based on the total weight of the cells and growth medium, and adjusting the pH to a level in the range of about 4 to about 6 that will permit flocculation, and then physically separating the 
     
     
        flocculated cells and the growth medium..]. .Iadd.10.  A process of recovering microbiologically produced cells from the medium in which they were produced comprising adding to the cell-containing medium gluten in an amount sufficient to cause flocculation at a pH in the range of 4 to 6, said addition of said gluten being made when said cell-containing medium is at a pH in the range of 4 to 6 that will permit flocculation, and then physically separating the flocculated cells from the medium in which they were produced. .Iaddend. .Iadd.11. A process according to claim 10 wherein the amount of said gluten is at least equal to about 0.2 percent of the 
     
     
        total weight of the cells and the growth medium. .Iaddend. .Iadd.12.  In the process of recovering microbiologically produced cellular protein products useful as animal food from the medium in which they were produced by adjusting the pH to a level in the range of 4 to 6 which will permit flocculation, adding a sufficient amount of a flocculating agent to cause flocculation, followed by physical separation, the improvement comprising utilizing gluten as the flocculating agent. .Iaddend. .Iadd.13. The process according to claim 12 wherein the amount of gluten employed in the recovery process is in the approximate range of from about 0.2 to about 25 weight percent of the total weight of the cells and growth medium. 
     
     
        .Iaddend. .Iadd.14.  The process according to claim 13 wherein the cells and the growth medium are at ambient temperature during the recovery 
     
     
        process. .Iaddend. .Iadd.15.  A process according to claim 13 wherein the flocculation is effected at a pH in the range of about 4 to about 5. .Iaddend. .Iadd.16. A process according to claim 15 wherein the 
     
     
        flocculation is effected at a pH of about 5. .Iaddend. .Iadd.17.  The method of recovering microbiologically produced cells at ambient temperature from the medium in which they were produced comprising adding to the medium gluten in an amount sufficient to cause flocculation at a pH in the range of 4 to 6, said amount being at least about 0.2 weight percent based on the total weight of the cells and growth medium, and adjusting the pH to a level in the range of 4 to 6 that will permit flocculation, and then physically separating the flocculated cells from the growth medium. .Iaddend.