Cell proliferation and tissue invasion inhibitor

Abstract

A composition of matter having activity as an inhibitor of cell proliferation .Iadd.and tissue invasion.Iaddend.is obtained by aqueous extraction of tissue having a high content of collagen and/or proteoglycans.

What is claimed is: 
     
       1. The method of preparing a composition of matter having activity as an inhibitor of cell proliferation .Iadd.and tissue invasion .Iaddend.comprising the steps of providing connective tissue having a high content of collagen or proteoglycans in condition for extraction, extracting said inhibitor from said tissue with an aqueous extraction medium which includes a solute which does not irreversibly denature the proteinaceous matter to be extracted, separating the resultant aqueous extract from the tissue, recovering from the aqueous extract substances having a molecular weight below about 50,000, treating the fraction of aqueous extract having a molecular weight below about 50,000 to remove salts therefrom, and dehydrating the resultant material. 
     
     
       2. The method of claim 1 wherein the connective tissue comprises tissue normally devoid of an intimate capillary blood supply. 
     
     
       3. The method of claim 2 wherein the tissue comprises cartilage. 
     
     
       4. The method of claim 2 wherein the tissue comprises blood vessels. 
     
     
       5. A composition of matter having activity as an inhibitor of cell proliferation .Iadd.and tissue invasion .Iaddend.comprising the product of the method of claim 1. 
     
     
       6. A composition of matter having activity as an inhibitor of cell proliferation comprising the product of the method of claim 2. 
     
     
       7. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 1. 
     
     
       8. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 2. 
     
     
       9. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 3. 
     
     
       10. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 4.