Process of preparing glucose isomerase

Abstract

.Iadd.Glucose is converted to fructose in the presence of an enzyme preparation derived from a member of the genus Arthrobacter. .Iaddend.

What is claimed is: .[.1. A process for preparing a glucose-isomerizing enzyme which comprises inoculating a carbohydrate-containing nutrient medium with a micro-organism belonging to the genus Arthrobacter, maintaining the inoculated medium for a period of time under conditions suitable for production of said enzyme by said organism and recovering said enzyme..]. .[.2. A process according to claim 1 in which said carbohydrate is xylose..]. .[.3. A process according to claim 1 in which said carbohydrate is xylan..]. .[.4. A process according to claim 1 in which said carbohydrate is glucose..]. .[.5. A process according to claim 2 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3724 or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or NRRL B-3728..]. .[.6. A process according to claim 3 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3724 or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or NRRL B-3728..]. .[.7. A process according to claim 4 in which the micro-organism in Arthrobacter nov. sp. NRRL B-3726 or NRRL B-3727 or NRRL 
     
        B-3728..]. .[.8.  A process for converting glucose to fructose which comprises incubating a glucose-containing liquor with a glucose-isomerizing enzyme derived from a micro-organism belonging to the genus Arthrobacter and recovering a fructose-containing liquor..]. .[.9. A process according to claim 8 in which said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing xylose..]. .[.10. A process according to claim 8 in which said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing xylan..]. .[.11. A process according to claim 8 in which said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing glucose..]. .[.12. A process according to claim 8 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing xylose or xylan..]. .[.13. A process according to claim 8 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from an Arthrobacter micro-organism grown in a glucose-containing nutrient medium in the absence of xylose or 
     
     
        xylan..]. .[.14.  A process according to claim 12 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3724 or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or NRRL B-3728..]. .[.15. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3726..]. .[.16. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3727..]. .[.17. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3728..]. .Iadd. 18. A process for preparing a glucose-isomerizing enzyme which comprises inoculating a carbohydrate-containing nutrient medium with an Arthrobacter microorganism capable of producing said enzyme in a nutrient medium containing glucose as the sole carbohydrate source, maintaining the inoculated medium for a period of time under conditions suitable for production of said enzyme by said organism and recovering said enzyme. .Iaddend..Iadd. 19. A process for converting glucose to fructose which comprises incubating a glucose-containing liquor with a glucose-isomerizing enzyme derived from an Arthrobacter microorganism capable of producing said enzyme in a nutrient medium containing glucose as the sole carbohydrate source and recovering a fructose-containing liquor. .Iaddend.