Process of preparing glucose isomerase

Abstract

Glucose is converted to fructose in the presence of an enzyme preparation derived from a member of the genus Arthrobacter. .Iadd.

What is claimed is: 
     
       1. A process for preparing a glucose-isomerizing enzyme which comprises inoculating a carbohydrate-containing nutrient medium with a micro-organism belonging to the genus Arthrobacter, maintaining the inoculated medium for a period of time under conditions suitable for production of said enzyme by said organism and recovering said enzyme. 
     
     
       2. A process according to claim 1 in which said carbohydrate is xylose. 
     
     
       3. A process according to claim 1 in which said carbohydrate is xylan. 
     
     
       4. A process according to claim 1 in which said carbohydrate is glucose. 
     
     
       5. A process according to claim 2 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3724 .[.or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or NRRL B-3728.].. 
     
     
       6. A process according to claim 3 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3724 .[.or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or NRRL B-3728.]..  .[.7. A process according to claim 4 in which the micro-organism in Arthrobacter nov. sp. NRRL B-3726 or NRRL B-3727 or 
     
     
        NRRL B-3728..]. 8. A process for converting glucose to fructose which comprises incubating a glucose-containing liquor with a glucose-isomerizing enzyme derived from a micro-organism belonging to the 
     
     
        genus Arthrobacter and recovering a fructose-containing liquor. 9. A process according to claim 8 in which said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing xylose. 
     
     
         0. A process according to claim 8 in which said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing xylan. 
     
     
          . A process according to claim 8 in which said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing glucose. 
     
     
        2. A process according to claim 8 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from an Arthrobacter micro-organism grown in a nutrient medium containing xylose 
     
     
        or xylan. 13. A process according to claim 8 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from an Arthrobacter micro-organism grown in a glucose-containing nutrient 
     
     
        medium in the absence of xylose or xylan. 14. A process according to claim 12 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3724 .[.or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or NRRL B-3728..]. .[.15. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3726..]. .[.16. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3727..]. .[.17. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3728..]. .Iadd. 18. A process for preparing a glucose-isomerizing enzyme which comprises inoculating a carbohydrate-containing nutrient medium with Arthrobacter nov. sp. NRRL B-3724, maintaining the inoculated medium for a period of time under conditions suitable for production of said enzyme by said organism and recovering said enzyme. .Iaddend..Iadd. 19. A process according to claim 18 in which said carbohydrate is xylose. .Iaddend. .Iadd. 20. A process for converting glucose to fructose which comprises incubating a glucose-containing liquor with a glucose-isomerizing enzyme derived from Arthrobacter nov. sp. NRRL B-3724 and recovering a fructose-containing liquor. .Iaddend..Iadd. 21. A process according to claim 20 in which said enzyme is derived from Arthrobacter nov. sp. NRRL B-3724 grown in a nutrient medium containing xylose. .Iaddend..Iadd. 22. A process according to claim 20 in which said enzyme is derived from Arthrobacter nov. sp. NRRL B-3724 grown in a nutrient medium containing glucose. .Iaddend..Iadd. 23. A process according to claim 20 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from Arthrobacter nov. sp. NRRL B-3724 grown in a nutrient medium containing xylose or xylan. .Iaddend.