Process of preparing glucose isomerase

Abstract

Glucose is converted to fructose in the presence of an enzyme preparation derived from a member of the genus Arthrobacter.

What is claimed is: 
     
       1. A process for preparing a glucose-isomerizing enzyme which comprises inoculating a carbohydrate-containing nutrient medium with .[.a micro-organism belonging to the genus Arthrobacter.]. .Iadd.the micro-organism Arthrobacter nov. sp. NRRL B-3725 or NRRL B-3728 .Iaddend.maintaining the inoculated medium for a period of time under conditions suitable for production of said enzyme by said organisms and recovering said enzyme. 
     
     
       2. A process according to claim 1 in which said carbohydrate is xylose. 
     
     
       3. A process according to claim 1 in which said carbohydrate is xylan. 
     
     
       4. A process according to claim 1 in which said carbohydrate is glucose. 
     
     
       5. A process according to claim 2 in which the micro-organism is Arthrobacter nov. sp. .[.NRRL B-3724 or.]. NRRL B-3725 .[.or NRRL B-3726 or NRRL B-3727 or NRRL B-3728.].. 
     
     
       6. A process according to claim 3 in which the micro-organism is Arthrobacter nov. sp. .[.NRRL B-3724 or.]. NRRL B-3725.[.or NRRL B-3726 or NRRL B-3727 or NRRL B-3728.].. 
     
     
       7. A process according to claim 4 in which the micro-organism .[.in.]. .Iadd.is .Iaddend.Arthrobacter nov. sp. .[.NRRL B-3726 or NRRL B-3727 or.].  NRRL B-3728. 
     
     
       8. A process for converting glucose to fructose which comprises incubating a glucose-containing liquor with a glucose-isomerizing enzyme derived from .[.a micro-organism belonging to the genus Arthrobacter.]. .Iadd.the micro-organism Arthrobacter nov. sp. NRRL B-3725 or NRRL B-3728 .Iaddend.and recovering a fructose-containing liquor. 
     
     
       9. A process according to claim 8 in which said enzyme is derived from .[.an.]. .Iadd.said .Iaddend.Arthrobacter micro-organism grown in a nutrient medium containing xylose. 
     
     
       10. A process according to claim 8 in which said enzyme is derived from .[.an.]. .Iadd.said .Iaddend.Arthrobacter micro-organism grown in a nutrient medium containing xylan. 
     
     
       11. A process according to claim 8 in which said enzyme is derived from .[.an.]. .Iadd.said .Iaddend.Arthrobacter micro-organism grown in a nutrient medium containing glucose. 
     
     
       12. A process according to claim 8 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from .[.an.]. .Iadd.said .Iaddend.Arthrobacter micro-organism grown in a nutrient medium containing xylose or xylan. 
     
     
       13. A process according to claim 8 in which the incubation is carried out at a temperature of about 50° C. to about 90° C. in a pH range of approximately 6.0 to 10.0 and said enzyme is derived from .[.an.]. .Iadd.said .Iaddend.Arthrobacter micro-organism grown in a glucose-containing nutrient medium in the absence of xylose or xylan. 
     
     
       14. A process according to claim 12 in which the micro-organism is Arthrobacter nov. sp. .[.NRRL B-3724 or NRRL B-3725 or NRRL B-3726 or NRRL B-3727 or.]. NRRL B-3728. .[.15. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3726..]. .[.16. A process according to claim 13 in which the micro-organism is Arthrobacter 
     
     
        nov. sp. NRRL B-3727..]. 17. A process according to claim 13 in which the micro-organism is Arthrobacter nov. sp. NRRL B-3728.