USH1531HExpiredUtility

Thermophilic DNA polymerase

69
Priority: Apr 18, 1994Filed: Apr 18, 1994Granted: May 7, 1996
Est. expiryApr 18, 2014(expired)· nominal 20-yr term from priority
Inventors:Ilse I. BlumentalsRoger LaskenBrian SchmidtMary LongoA. John Hughes, Jr.Deb K. Chatterjee
C12N 9/1252
69
PatentIndex Score
32
Cited by
45
References
2
Claims

Abstract

The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
       1. A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2. 
     
     
       2. A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2 except wherein asparagine at position 141 has been replaced by alanine and glutamine at position 143 has been replaced by alanine.

Join the waitlist — get patent alerts

Track USH1531H — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.