RNAi targeting SEC61G

Abstract

Efficient sequence specific gene silencing is possible through the use of RNAi technology. By selecting particular RNAi molecules by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of RNAi molecules are disclosed including those directed to nucleotide sequences for SEC61G.

We claim: 
     
       1. An RNA molecule capable of inducing the RNA interference pathway, wherein said RNA molecule consists of: (a) a duplex region; and (b) either no overhang regions or at least one overhang region, wherein each overhang region contains six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said sense region and said antisense region are each 19-30 nucleotides in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 448. 
     
     
       2. The RNA molecule of  claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length. 
     
     
       3. The RNA molecule of  claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length. 
     
     
       4. The RNA molecule of  claim 1 , wherein said RNA molecule has at least one overhang region. 
     
     
       5. The RNA molecule of  claim 1 , wherein said RNA molecule has no overhang regions. 
     
     
       6. A chemically synthesized double stranded RNA molecule, wherein:
 (a) each strand of said double stranded RNA molecule is between 19 and 30 nucleotides in length; and 
 (b) one strand of said double stranded RNA molecule comprises a sequence that is the complement of SEQ ID NO: 448. 
 
     
     
       7. The chemically synthesized double stranded RNA molecule of  claim 6  wherein each strand of said double stranded RNA molecule is 19-25 nucleotides in length. 
     
     
       8. The chemically synthesized double stranded RNA molecule of  claim 7 , wherein each strand of said double stranded RNA molecule is 19 nucleotides in length. 
     
     
       9. The RNA molecule of  claim 6 , wherein said double stranded RNA molecule has at least one overhang region. 
     
     
       10. The RNA molecule of  claim 6 , wherein said double stranded RNA molecule has no overhang regions. 
     
     
       11. A pool of at least two RNA molecules capable of inducing the RNA interference pathway, wherein said pool comprises a first RNA molecule and a second RNA molecule, wherein said first RNA molecule is the RNA molecule of  claim 1 , wherein the duplex region of said first RNA molecule is a first duplex region and said second RNA molecule consists of a second duplex region and either no overhang regions or at least one overhang region, wherein each overhang region contains six or fewer nucleotides, wherein the second duplex region of said second RNA molecule comprises a sense region and an antisense region, wherein said sense region and said antisense region of said second RNA together form said second duplex region and said sense region and said antisense region of said second duplex region are each 19-30 nucleotides in length and said antisense region of said second duplex region comprises a sequence that is the complement of a sequence selected from the group consisting of SEQ ID NO: 438-447 and 449-488. 
     
     
       12. The pool of  claim 11 , wherein said first RNA molecule and said second RNA molecule each has no overhang regions. 
     
     
       13. The pool of  claim 11 , wherein said antisense region and said sense region of said first RNA molecule and said antisense region and said sense region of said second RNA molecule are each 19-25 nucleotides in length. 
     
     
       14. The pool of  claim 13 , wherein said antisense region and said sense region of said first RNA molecule and said antisense region and said sense region of said second RNA molecule are each 19 nucleotides in length. 
     
     
       15. The pool of  claim 12 , wherein said first RNA molecule contains no overhang regions. 
     
     
       16. The pool of  claim 13 , wherein the second RNA molecule contains no overhang regions. 
     
     
       17. A method for inhibiting the expression of a gene in a cell, the method comprising introducing into the cell in vitro the RNA molecule of  claim 1 , wherein the gene is SEC61G. 
     
     
       18. The method according to  claim 17 , wherein the cell is a human cell. 
     
     
       19. The method according to  claim 17 , wherein said introducing is via transfection. 
     
     
       20. The method according to  claim 17 , wherein said introducing is via passive uptake.