Detection and decomposition of bisphenol-A

Abstract

An enzyme electrode that enables rapid degradation of bisphenol-A (BPA) in a contaminated sample and/or monitoring of BPA concentration in a contaminated sample. The enzyme electrode includes a working electrode having one or more selected enzymes that are capable of degrading BPA linked to the working electrode. The selected enzymes linked to the working electrode are capable of degrading BPA at an enhanced rate in response to an applied voltage. The electrode can be used to monitor BPA concentration in a contaminated sample by measuring current flow through the electrode in response to an applied voltage.

What is claimed is:   
     
       1. An enzyme electrode system configured for detecting and/or degrading bisphenol-A (BPA), comprising:
 a building water reservoir configured to receive and dispense drinking water; 
 the enzyme electrode comprising:
 a working electrode; 
 a porous silica Gel film linked to the working electrode; 
 a electropolymerized film material linked to the porous silica gel film; 
 an enzyme configured to react with BPA linked to the working electrode by being located in the electropolymerized film material, wherein the enzyme includes one or more of manganese peroxidase (MnP), lignin peroxidase, or versatile peroxidase; and 
 a coenzyme and/or enzyme mediator contained in the electropolymerized film material and operatively coupled to the working electrode and the enzyme, wherein the coenzyme and/or enzyme mediator is selected from the group consisting of1-hydroxybenzotriazole (HBT), a ferrocene compound, tetrathiofulvalene (TTF), an osmium complex, an organic dye, a phthalocyanine, ferrocyanide, a polypyrrole, 7,7,8,8-tetracyanoquinodimethane (TCNQ), NADH, NADPH, FAD, FADPH, and combinations thereof; 
 wherein the enzyme electrode system is configured to operate at a voltage of about +0.25V to about +1 V for detecting and/or degrading BPA; and 
 wherein the enzyme electrode is positioned in the building water reservoir so as to detect and/or degrade the BPA in the drinking water in the building water reservoir. 
 
 
     
     
       2. The enzyme electrode system of  claim 1 , comprising:
 an electrically insulating base plate; 
 the working electrode located on the insulating base plate; and 
 a counter electrode located on the insulating base plate separated from the working electrode. 
 
     
     
       3. The enzyme electrode system of  claim 2 , further comprising a reference electrode located on the insulating base plate separated from the working electrode and/or the counter electrode. 
     
     
       4. The enzyme electrode system of  claim 1 , wherein the electrode of the working electrode includes a carbon electrode. 
     
     
       5. The enzyme electrode system of  claim 1 , wherein the electropolymerized film includes one or more of poly(1,3-phenyl diamine), polythiophene, polyphenol, polyaniline, or poly(1,2-phenyl diamine). 
     
     
       6. The enzyme electrode system of  claim 1 , wherein the working electrode includes an electrically conductive mesh layer configured for increasing the surface area of the working electrode. 
     
     
       7. The enzyme electrode system of  claim 1 , further comprising an entrapment layer configured to at least partially maintain linkage between the enzyme and the working electrode. 
     
     
       8. The enzyme electrode system of  claim 1 , wherein the building water reservoir includes a water faucet, the enzyme electrode being in fluid contact with water flowing through the water faucet so as to detect and/or degrade BPA in the water flowing therethrough. 
     
     
       9. The enzyme electrode system of  claim 8 , wherein the enzyme electrode is disposed in a water flow path of the water faucet so as to detect and/or degrade BPA in water flowing through the faucet.