Adhesive hemostatic agent based on porcine atelocollagen and method for production thereof

Abstract

Disclosed is an adhesive hemostatic agent based on non-blood constituents including DOPA, able to strongly adhere to collagen fibers, and which comprises an antifibrinolytic agent in addition to an esterified atelocollagen which is non-immunogenic and may become positively charged thereon such that the adhesive hemostatic agent has no possibility of mediating particular diseases or viral infections (HIV, HCV, HBV, CMV, etc), unlike conventional agents comprising blood constituents, and readily binds to negatively charged platelets at high adhesive strength, thus inducing quick blood coagulation. Also, provided is a method for preparing the same.

I claim: 
     
       1. A method for preparing a collagen-based adhesive hemostatic agent, comprising:
 (a) removing immunogenic telo-peptides from porcine collagen through enzyme treatment to afford an atelocollagen; 
 (b) reacting an aqueous solution of the atelocollagen with an ethanol solution to afford an esterified atelocollagen; 
 (c) adding 3,4-dihydroxy-phenyl alanine to an aqueous solution containing calcium chloride; 
 (d) mixing an aqueous solution containing the esterified atelocollagen with the aqueous solution obtained by the step (c); and 
 wherein the 3,4-dihydroxy-phenyl alanine is oxidized into catecholamine which is associated with a lysine moiety of the esterified atelocollagen, so as to provide adhesiveness to the hemostatic agent. 
 
     
     
       2. The method as defined in  claim 1 , wherein an antifibrinolytic is further added to the aqueous solution obtained by the step (c). 
     
     
       3. The method as defined in  claim 2 , wherein the antifibrinolytic is tranexamic acid or aminocaproic acid.