Double-stranded nucleic acid molecule cancer cell proliferation inhibitor and pharmaceutical agent suitable for prevention or treatment of uterine cancer, breast cancer, and bladder cancer

Abstract

A double-stranded nucleic acid molecule for suppressing the expression of at least one of COX7RP and Efp genes which includes (a) a sense strand which includes a nucleotide sequence corresponding to a target sequence indicated by any one of SEQ ID Nos.: 1 to 38 and (b) an antisense strand which includes a nucleotide sequence complementary to that of the sense strand specified in (a); a cancer cell proliferation inhibitor with the double-stranded nucleic acid molecule and against at least one of uterine, breast and bladder cancer cells; and a pharmaceutical agent with the cancer cell proliferation inhibitor and against at least one of uterine, breast and bladder cancers.

What is claimed is: 
     
       1. A double-stranded nucleic acid molecule comprising:
 (a) a sense strand which comprises a nucleotide sequence corresponding to a target sequence indicated by any one of SEQ ID Nos.: 1 to 38, and 
 (b) an antisense strand which comprises a nucleotide sequence complementary to that of the sense strand specified in (a), 
 
       wherein the double-stranded nucleic acid molecule is for suppressing the expression of at least one of COX7RP and estrogen-responsive finger protein (Efp) genes. 
     
     
       2. The double-stranded nucleic acid molecule according to  claim 1 , wherein the sense strand has a nucleotide sequence which corresponds to a target sequence indicated by any one of SEQ ID Nos.: 1 to 5 and 16 to 21. 
     
     
       3. The double-stranded nucleic acid molecule according to  claim 2 , wherein the sense strand has a nucleotide sequence which corresponds to a target sequence indicated by any one of SEQ ID Nos.: 1, 2, 4, 5, 16, 18, 19, 20 and 21. 
     
     
       4. The double-stranded nucleic acid molecule according to  claim 3 , wherein the sense strand has a nucleotide sequence which corresponds to a target sequence indicated by any one of SEQ ID Nos.: 1, 2 and 16. 
     
     
       5. The double-stranded nucleic acid molecule according to  claim 4 , wherein the sense strand has a nucleotide sequence which corresponds to a target sequence indicated by any one of SEQ ID Nos.: 2 and 16. 
     
     
       6. The double-stranded nucleic acid molecule according to  claim 1 , wherein the double-stranded nucleic acid molecule is at least one of a double-stranded RNA and a double-stranded RNA-DNA chimera. 
     
     
       7. The double-stranded nucleic acid molecule according to  claim 6 , wherein the double-stranded nucleic acid molecule is at least one of siRNA and chimera siRNA. 
     
     
       8. DNA comprising:
 a nucleotide sequence encoding a double-stranded nucleic acid molecule which comprises
 (a) a sense strand which comprises a nucleotide sequence corresponding to a target sequence indicated by any one of SEQ ID Nos.: 1 to 38, and 
 (b) an antisense strand which comprises a nucleotide sequence complementary to that of the sense strand specified in (a), 
 
 
       wherein the double-stranded nucleic acid molecule is for suppressing the expression of at least one of COX7RP and estrogen-responsive finger protein (Efp) genes. 
     
     
       9. A vector comprising:
 DNA which comprises a nucleotide sequence encoding a double-stranded nucleic acid molecule which comprises
 (a) a sense strand which comprises a nucleotide sequence corresponding to a target sequence indicated by any one of SEQ ID Nos.: 1 to 38, and 
 (b) an antisense strand which comprises a nucleotide sequence complementary to that of the sense strand specified in (a), 
 
 
       wherein the double-stranded nucleic acid molecule is for suppressing the expression of at least one of COX7RP and estrogen-responsive finger protein (Efp) genes. 
     
     
       10. A cancer cell proliferation inhibitor comprising:
 at least one of a double-stranded nucleic acid molecule which comprises
 (a) a sense strand which comprises a nucleotide sequence corresponding to a target sequence indicated by any one of SEQ ID Nos.: 1 to 38, and 
 (b) an antisense strand which comprises a nucleotide sequence complementary to that of the sense strand specified in (a), 
 
 wherein the double-stranded nucleic acid molecule is for suppressing the expression of at least one of COX7RP and estrogen-responsive finger protein (Efp) genes; DNA which comprises a nucleotide sequence encoding the double-stranded nucleic acid molecule; and a vector which comprises the DNA, 
 wherein the cancer cell proliferation inhibitor is for inhibiting the growth of at least one of uterine cancer cells, breast cancer cells and bladder cancer cells. 
 
     
     
       11. A pharmaceutical agent comprising:
 a cancer cell proliferation inhibitor which comprises at least one of a double-stranded nucleic acid molecule which comprises
 (a) a sense strand which comprises a nucleotide sequence corresponding to a target sequence indicated by any one of SEQ ID Nos.: 1 to 38, and 
 (b) an antisense strand which comprises a nucleotide sequence complementary to that of the sense strand specified in (a), 
 
 wherein the double-stranded nucleic acid molecule is for suppressing the expression of at least one of COX7RP and estrogen-responsive finger protein (Efp) genes; DNA which comprises a nucleotide sequence encoding the double-stranded nucleic acid molecule; and a vector which comprises the DNA, 
 wherein the cancer cell proliferation inhibitor is for inhibiting the growth of at least one of uterine cancer cells, breast cancer cells and bladder cancer cells, and 
 wherein the pharmaceutical agent is for treating at least one of uterine cancer, breast cancer and bladder cancer.