Method for in vitro mass culture of Aloe vera

Abstract

The present invention provides a method for producing a true-to-type clone of an Aloe bαrbαdensis mother plant by selecting an Aloe bαrbαdensis mother plant; isolating a meristematic explant from the plant; culturing the meristematic explant in initiation medium to generate shoots, where the media lacks hormones; culturing the shoots in proliferation and elongation medium to generate elongated shoots, where the proliferation and elongation media comprises benzyl adenine (BA) and indole butyric acid (IBA); culturing the elongated shoots in rooting medium to generate plantlets, where the media lacks hormones; and culturing the plantlets to product a true-to-type clone of the Aloe bαrbαdensis mother plant.

1. A method for producing a true-to-type clone of an  Aloe barhadensis  mother plant having acemannan content between 9.9% and 12.1% in a hormone free initiation and rooting medium comprising the steps of:
 a) isolating a meristematic explant from said mother plant; 
 b) culturing said meristematic explant in a hormone free initiation medium to generate shoots; 
 c) culturing said shoots in proliferation and elongation medium comprising benzyladenine (BA) and indole butyric acid (IBA) to generate elongated shoots; 
 d) culturing said elongated shoots in a hormone free MS solidified rooting medium to generate plantlets; and 
 e) culturing said plantlets to produce a true-to-type clone of said  Aloe barbadensis  mother plant. 
 
     
     
       2. The method of  claim 1 , wherein said benzyl adenine is at a concentration selected from the group consisting of: 1.5 mg/L, 2 mg/L, 2.5 mg/L, and 3 mg/L. 
     
     
       3. The method of  claim 2 , wherein said benzyl adenine is at a concentration of about 1.5 mg/L. 
     
     
       4. The method of  claim 1 , wherein said indole butyric acid is at a concentration selected from the group consisting of: 0.15 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L, and 3 mg/L. 
     
     
       5. The method of  claim 4 , wherein said indole butyric acid is at a concentration of about 0.15 mg/L. 
     
     
       6. The method of  claim 1 , wherein said meristematic explant is from a shoot tip or a nodal bud. 
     
     
       7. The method of  claim 4 , wherein said meristematic explant is from a shoot tip.