Anti-ulcer pharmaceutical composition and the preparation thereof

Abstract

The invention discloses a pharmaceutical composition for preventing and/or treating peptic ulcer, including American ginseng or the extract thereof, and a method for preparing American ginseng extract, said method including extracting American ginseng with water or ethanol aqueous solution, and then ultrafiltrating, dialyzing, precipitating with ethanol, or performing reverse phase chromatography to obtain various fractions of extract with anti-peptic ulcer effect.

What is claimed is: 
     
       1. A method for preventing and/or treating a patient suffering from peptic ulcer, comprising administering an effective amount of an American ginseng extract to said patient, wherein the American ginseng extract is obtained by:
 extracting American ginseng with a solvent having a polarity higher than 0.88 to obtain an extract; 
 filtering said extract to obtain a filtrate; 
 centrifuging said filtrate and collecting a supernatant; and 
 separating said supernatant with a molecular weight cut-off of at least 1,000 to give a fraction with molecular weight of greater than 1,000, and said fraction being concentrated to give said American ginseng extract, 
 
       wherein the American ginseng extract contains compounds with molecular weights of at least 3,000. 
     
     
       2. The method as claimed in  claim 1 , wherein the solvent is water or a 10%~80% aqueous ethanol solution. 
     
     
       3. The method as claimed in  claim 1 , wherein the separating step is conducted by an ultrafiltration membrane having a molecular weight cut-off of 1,000. 
     
     
       4. The method as claimed in  claim 1 , wherein the separating step is conducted by an ultrafiltration membrane having a molecular weight cut-off of 3,000. 
     
     
       5. The method as claimed in  claim 1 , wherein the solvent is a 10%~8% aqueous ethanol solution; and the American ginseng extract further comprises a composition obtained by separating said supernatant with a molecular weight cut-off of at least 1,000 to give a solution containing substances having molecular weights of less than 1,000 dalton, the solution being concentrated to dry and mixed with an ethanol solution to dissolve said substances, and filtering said ethanol solution to obtain a soluble portion as a part of said American ginseng extract. 
     
     
       6. The method as claimed in  claim 1 , wherein the solvent is a 10%~80% aqueous ethanol solution; the separating step is conducted by an ultrafiltration membrane with a molecular weight cut-off of 3,000; and the American ginseng extract further comprises a composition obtained by separating said supernatant with a molecular weight cut-off of 3,000 to give a solution containing substances having molecular weights of less than 3,000 dalton, loading the solution onto a reverse phase column, eluting the column with water and a 95% ethanol solution, and collecting a 95% ethanol eluate as a part of said American ginseng extract. 
     
     
       7. A method for preventing and/or treating a patient suffering from peptic ulcer, comprising administrating an effective amount of American ginseng extract to said patient, wherein the American ginseng extract is obtained by:
 extracting American ginseng with a solvent with a polarity higher than 0.88 to obtain an extract; 
 filtering said extract to obtain a filtrate; 
 centrifuging said filtrate and collecting a supernatant; and 
 separating said supernatant with molecular weight cut off at least 3,000 to give a fraction with molecular weight greater than 3,000, and said fraction being concentrated to give said American ginseng extract. 
 
     
     
       8. The method as claimed in  claim 7 , wherein the solvent is water or 10%–80% aqueous ethanol solution. 
     
     
       9. The method as claimed in  claim 7 , wherein separating said supernatant with molecular weight cut off at least 3,000 is using an ultrafiltration membrane with molecular weight cut off 3,000. 
     
     
       10. The method as claimed in  claim 7 , wherein the solvent is 10%–80% aqueous ethanol solution, separating said supernatant with molecular weight cut off at least 3,000 is using an ultrafiltration membrane with molecular weight cut off 3,000 and the American ginseng extract further comprises a composition obtained by separating said supernatant with molecular weight cut 3,000 to give a filtrate containing substances with molecular weight less than 3,000 dalton, the filtrate being loaded onto a reverse phase column, eluted with water, followed by 95% ethanol solution and collected the 95% ethanol eluate as a part of said American ginseng extract.