Methods for using lipases in baking

Abstract

The present invention relates to methods for preparing a dough, comprising incorporating into the dough a composition comprising an effective amount of a lipase which improves one or more properties of the dough or a baked product obtained from the dough. The present invention also relates to methods for preparing a baked product. The present invention also relates to compositions comprising an effective amount of such a lipase for improving one or more properties of a dough and/or a baked product obtained from the dough. The present invention further relates to doughs or baked products and to pre-mixes for a dough.

What is claimed is:  
     
       1. A pre-mix for a dough comprising a baking agent and an effective amount of a lipase selected from the group consisting of: 
       (a) a lipase having an amino acid sequence which has at least 85% identity with amino acids 31 to 349 of SEQ ID NO:2;  
       (b) a lipase encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleotides 1525 to 2530 of SEQ ID NO:1, (ii) the cDNA sequence contained in nucleotides 1525 to 2530 of SEQ ID NO:1, or (iii) a complementary strand of (i) or (ii); and  
       (c) a fragment of (a) or (b), which has lipase activity.  
     
     
       2. The pre-mix of  claim 1 , wherein the lipase has an amino acid sequence which has at least 85% identity with amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       3. The pre-mix of  claim 2 , wherein the lipase has an amino acid sequence which has at least 90% identity with amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       4. The pre-mix of  claim 3 , wherein the lipase has an amino acid sequence which has at least 95% identity with amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       5. The pre-mix of  claim 1 , wherein the lipase comprises the amino acid sequence of SEQ ID NO:2. 
     
     
       6. The pre-mix of  claim 1 , wherein the lipase consists of the amino acid sequence of SEQ ID NO:2 or a fragment thereof which has lipase activity. 
     
     
       7. The pre-mix of  claim 6 , wherein the lipase consists of the amino acid sequence of SEQ ID NO:2. 
     
     
       8. The pre-mix of  claim 7 , wherein the lipase consists of amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       9. The pre-mix of  claim 1 , wherein the lipase is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleotides 1525 to 2530 of SEQ ID NO:1, (ii) the cDNA sequence contained in nucleotides 1525 to 2530 of SEQ ID NO:1, or (iii) a complementary strand of (i) or (ii). 
     
     
       10. The pre-mix of  claim 1 , wherein the lipase is encoded by the nucleic acid sequence contained in plasmid pEJG60 which is contained in  E. coli  NRRL B30333. 
     
     
       11. A baking additive in the form of a granulate or agglomerated powder, comprising an effective amount of a lipase selected from the group consisting of. 
       (a) a lipase having an amino acid sequence which has at least 85% identity with amino acids 31 to 349 of SEQ ID NO:2;  
       (b) a lipase encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleoides 1525 to 2530 of SEQ ID NO:1, (ii) the cDNA sequence contained in nucleotides 1525 to 2530 of SEQ ID NO:1, or (iii) a complementary strand of (i) or (ii); and  
       (c) a fragment of (a) or (b), which has lipase activity.  
     
     
       12. The baking additive of  claim 11 , wherein the lipase has an amino acid sequence which has at least 85% identity with amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       13. The baking additive of  claim 12 , wherein the lipase has an amino acid sequence which has at least 90% identity with amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       14. The baking additive of  claim 13 , wherein the lipase has an amino acid sequence which has at least 95% identity with amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       15. The baking additive of  claim 11 , wherein the lipase comprises the amino acid sequence of SEQ ID NO:2. 
     
     
       16. The baking additive of  claim 11 , wherein the lipase consists of the amino acid sequence of SEQ ID NO:2 or a fragment thereof which has lipase activity. 
     
     
       17. The baking additive of  claim 16 , wherein the lipase consists of the amino acid sequence of SEQ ID NO:2. 
     
     
       18. The baking additive of  claim 17 , wherein the lipase consists of amino acids 31 to 349 of SEQ ID NO:2. 
     
     
       19. The baking additive of  claim 11 , wherein the lipase is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleotides 1525 to 2530 of SEQ ID NO:1, (ii) the cDNA sequence contained in nucleotides 1525 to 2530 of SEQ ID NO:1, or (ii) a complementary strand of (i) or (ii). 
     
     
       20. The baking additive of  claim 11 , wherein the lipase is encoded by the nucleic acid sequence contained in plasmid pEJG60 which is contained in  E. coli  NRRL B30333.