Beta 2 adrenergic polymorphism detection

Abstract

Nucleic acid sequences are provided that are useful as amplification primers, hybridization probes, and as a portion of molecular beacon probes for amplifying and detecting polymorphisms of the beta2 adrenergic receptor gene, compositions and kits incorporating the same, and methods employing the same.

What is claimed is:  
     
       1. A composition comprising a molecular beacon probe for detecting a target sequence, the molecular beacon probe comprising a label moiety and a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO:7, SEQ ID NO: 8, and SEQ ID NO: 9. 
     
     
       2. The composition of  claim 1 , wherein the molecular beacon probe comprises a label moiety and a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 6 and SEQ ID NO: 9. 
     
     
       3. The composition of  claim 1 , further comprising a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO 2, and SEQ ID NO 3. 
     
     
       4. A method for detecting a target sequence in a test sample comprising the steps of: 
       (a) forming a reaction mixture comprising nucleic acid amplification reagents, a nucleic acid having the nucleotide sequence designated SEQ ID NO:2, a nucleic acid having the nucleotide sequence designated SEQ ID NO:3, and a test sample suspected of containing a target sequence;  
       (b) subjecting the mixture to amplification conditions to generate an amplification product;  
       (c) contacting the amplification product with a molecular beacon probe comprising a label moiety and a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO:7, SEQ ID NO: 8 and SEQ ID NO: 9 such that in the presence of the target sequence the molecular beacon probe generates a signal; and  
       (d) detecting the signal generated by the beacon probe as an indication of the presence of the target sequence in the test sample.  
     
     
       5. The method of  claim 4 , wherein the molecular beacon probe therefore comprises a label moiety and a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 6, and SEQ ID NO: 9. 
     
     
       6. A kit for amplifying a β2 adrenergic receptor target sequence comprising: 
       (a) a nucleic acid having the nucleotide sequence of SEQ ID NO: 2 and a nucleic acid having the nucleotide sequence of SEQ ID NO: 3;  
       (b) amplification reagents; and  
       (c) a molecular beacon probe comprising a label moiety and a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9.  
     
     
       7. The kit of  claim 6 , wherein the molecular beacon probe therefore comprises a label moiety and a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 6 and SEQ ID NO: 9.