High-resolution NMR spectroscopy of molecules encapsulated in low-viscosity fluids

Abstract

The present invention provides a method for reducing the correlation time for global tumbling for analysis of proteins and other macromolecules by NMR spectroscopy. The method comprises placing a macromolecule, hydrated and without significant structure perturbation, within a reverse micelle in a suitable fluid medium of low viscosity. A high pressure NMR cell for use with the method of the present invention is also disclosed.

We claim:  
     
       1. A Pressure cell for use in NMR spectroscopy of molecules comprising: 
       (a) a sample tube;  
       (b) a housing for holding the sample tube; and  
       (c) a cover for closing the housing, said cover, having at least one opening for inflow of fluid and at least one opening for outflow of fluid,  
       wherein the sample tube self-seals under pressure against the housing and wherein the cover self-seals under pressure against the housing.  
     
     
       2. The pressure cell of  claim 1 , wherein the sample tube self-seals under pressure against the housing by an O-ring and a washer. 
     
     
       3. The pressure cell of  claim 1 , wherein the cover self-seals under pressure against the housing by an O-ring.