Process for the expression and production of the recombinant growth factor protein (BEGF) derived from BEAN 58058 virus (BEV)

Abstract

The present invention describes recombinant growth factor (BEGF) protein isolated from the virus BeAn58058, their corresponding encoding recombinant DNA molecule and the process of production of the recombinant BEGF protein produced through genetic engineering techniques, to be used in human or veterinarian clinical or in research.

What is claimed is:  
     
       1. A process for preparing a BEGF polypeptide, comprising: 
       introducing a nucleic acid encoding for a protein of SEQ ID NO:5 into a host cell, and  
       culturing said cell under conditions suitable to effect expression of said nucleic acid and to produce said polypeptide.  
     
     
       2. The process according to  claim 1 , further comprising isolating said peptide. 
     
     
       3. The process according to  claim 1 , wherein said polypeptide is isolated by applying said polypeptide to a nickel chelate resin. 
     
     
       4. The process according to  claim 1 , wherein said host cell is a bacterium. 
     
     
       5. The process according to  claim 4 , wherein said bacterium is  Escherlchia coli.    
     
     
       6. A process for preparing a BEGF polypeptide, comprising: 
       introducing a nucleic acid encoding for a protein of SEQ ID NO: 5 into a host cell, said host cell being  E.coli,    
       culturing said cell under conditions suitable to effect expression of said nucleic acid and to produce said polypeptide, and  
       isolating said polypeptide by applying said polypeptide to a nickel chelate resin.  
     
     
       7. An isolated protein comprising the amino acid sequence of SEQ ID NO: 5. 
     
     
       8. The isolated protein according to  claim 7 , consisting of the amino acid sequence of SEQ ID NO: 5.