US5756285AExpiredUtility

DNA cycle sequencing

Assignee: AMERSHAM LIFE SCIENCE INCPriority: Sep 27, 1991Filed: Apr 14, 1994Granted: May 26, 1998

Est. expirySep 27, 2011(expired)· nominal 20-yr term from priority

Inventors:Carl W. Fuller

C12Q 1/6869

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References

Claims

Abstract

Method for determining the nucleotide base sequence of a DNA molecule by amplifying that DNA, contacting the products of the amplifying reaction with an alkaline phosphatase, and determining the nucleotide base sequence of any polynucleic acid products of the amplifying reaction.

Claims

exact text as granted — not AI-modified

I claim: 
     
       1. A method for determining the nucleotide base sequence of a DNA molecule, comprising the steps of amplifying said DNA by a polymerase chain reaction, contacting the products of said amplifying step with an alkaline phosphatase, and determining the nucleotide base sequence of any polynucleic acid products of said amplifying step by a chain termination procedure. 
     
     
       2. The method of claim 1, wherein the products of said amplifying step are contacted with exonuclease I.

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