Split feed cell fermentation system

Abstract

Provided are methods for fed batch fermentation of cells to produce a compound of interest. Also provided are methods of preparing a compound of interest.

We claim: 
     
       1. A method for fed batch culturing of cells in a fermentor comprising independently introducing into the fermentor during the growth and production phases of culturing: a) a carbon nutrient source from a first feed vessel; and   b) a nitrogen nutrient source from a second feed vessel.   
     
     
       2. The method of claim 1 wherein the carbon and nitrogen nutrient sources are independently introduced into the fermentor by a rate control means. 
     
     
       3. The rate control means of claim 2 that is a pump. 
     
     
       4. The method of claims 1, 2, or 3 wherein the cells are bacteria cells. 
     
     
       5. A method for preparing a compound of interest comprising fed batch culturing of host cells that produce the compound wherein a carbon nutrient source is introduced to the fermentor from a first feed vessel and a nitrogen nutrient source is introduced to the fermentor form a second feed vessel, and wherein the nutrient sources are delivered to the fermentor at independent rates during both the growth and production phases of culturing. 
     
     
       6. The method of claim 5 wherein the carbon and nitrogen nutrient sources are independently introduced to the fermentor by a rate control means. 
     
     
       7. The method of claim 5 wherein the rate control means is a pump.