Process for the production of lignolytic enzymes by means of white rot fungi

Abstract

The present invention relates to a process for the production of lignolytic enzymes by means of white rot fungi. In the process, a culture composed of the fungi of the subclass of the Aphyllophorales is placed in a closed fermentation vessel filled with a nutrient solution and the suspension is gently moved for the production of pellets of Aphyllophorales, such that there cannot be any shearing forces which would destroy the pellets, wherein the enzymes are produced with the addition of yeasts or benzaldehydes, chlorobenzaldehydes, nitrobenzaldehydes, hydroxybenzaldehydes, aminobenzaldehydes, methylbenzaldehydes, diaryls or triaryls, dialkylalkanes, trialkyl alkanes, open-chained or cyclic imines or derivatives of the aforementioned substances as inductive compounds, wherein the induction may take place one time or several times, and the enzymes are subsequently harvested.

I claim: 
     
       1. Process for the production of lignolytic enzymes by means of white rot fungi, wherein a culture composed of the fungi of the subclass of the Aphyllophorales is placed in a closed fermentation vessel filled with a nutrient solution and the suspension is gently moved for the production of pellets of Aphyllophorales, such that there cannot be any shearing forces which would destroy the pellets, the process comprising producing the enzymes with the addition of yeasts or benzaldehydes, chlorobenzaldehydes, nitrobenzaldehydes, hydroxybenzaldehydes, aminobenzaldehydes, methylbenzaldehydes, diaryls or triaryls, dialkylalkanes, trialkyl alkanes, open-chained or cyclic imines or derivatives of the aforementioned substances as inductive compounds, wherein the induction may take place one time or several times, and wherein the enzymes are subsequently harvested.   
     
     
       2. Process according to claim 1, wherein after the conclusion of the enzyme production, the pellets are separated from the liquid nutrient medium and are returned again into the fementer for the enzyme production. 
     
     
       3. Process according to claim 1, wherein the culture is placed into a fermenter equipped with a stirrer whose paddles can be adjusted, such that no shearing forces occur which destroy the pellets. 
     
     
       4. Process according to claim 1, wherein Phanerochaete chrysosporium with the addition of yeasts is placed into the fermentation vessel. 
     
     
       5. Process according to claim 1, wherein Coriolus versicolor produces the enzymes with the addition of benzaldehydes, chlorobenzaldehydes, nitrobenzaldehydes, hydroxybenzaldehydes, aminobenzaldehydes, methylbenzaldehydes, diaryls or triaryls, dialkylalkanes, trialkyl alkanes, open-chained or cyclic imines or derivatives of the aforementioned substances as inductive compounds, wherein the induction may take place one time or several times.