Biological assembly

Abstract

A biological assembly for effecting the examination of a sample in a liquid medium under magnification. The assembly includes a transparent slide with a sample chamber and an immediately adjacent overflow chamber formed on one main surface by a thin film. The sample chamber is receptive of a sample in a liquid medium. transparent slide, having a pattern for aligning it with the sample chamber, is disposed over the sample chamber. The transparent slide includes a grid etched into the lower surface thereof to enable the counting of cells under magnification.

What is claimed is: 
     
       1. A biological assembly for effecting the examination of a sample in a liquid medium under magnification, comprising: a transparent substrate having a first main surface;   means forming at least one sample chamber and an immediately adjacent overflow chamber on the first main surface and receptive of a sample in a liquid medium, said means comprising a thin film having for each sample chamber and overflow chamber, means defining a first opening therein corresponding to the sample chamber and means defining a second opening surrounding the first opening and corresponding to the overflow chamber, wherein the first and second openings form a separating wall of thin film therebetween; and   a transparent cover having first main surface superposable on the thin film to cover the at least one sample chamber and overflow chamber when a sample is received therein, wherein the cover has a second main surface opposite the first main surface and having a thin film pattern thereon demarcating an area for aligning the area of the cover with the at least one sampling chamber.   
     
     
       2. The biological assembly according to claim 1, wherein the first opening is circular and the second opening is toroidal. 
     
     
       3. The biological assembly according to claim 1, wherein the first opening is circular and the second opening is rectangular. 
     
     
       4. The biological assembly according to claim 1, wherein the thin film has a height of from 10 to 50 μm. 
     
     
       5. The biological assembly according to claim 1, wherein the separating wall has a width of 200 to 800 μm. 
     
     
       6. The biological assembly according to claim 1, wherein said at least one sample chamber has a volume of 2 to 15 μl. 
     
     
       7. The biological assembly according to claim 1, wherein the thin film pattern is identical in configuration to said separating wall. 
     
     
       8. The biological assembly according to claim 1, wherein the cover has a grid pattern in the first main surface within the area demarcated by said thin film pattern. 
     
     
       9. The biological assembly according to claim 1, wherein the thin film comprises at least one of a photoresist, an epoxy and a hydrophobic ink. 
     
     
       10. A biological assembly for effecting examination of a sample in a liquid medium under magnification, comprising: a transparent substrate having a first main surface;   means forming at least one sample chamber on the first main surface and receptive of a sample in a liquid medium, said means comprising a thin film having for each sample chamber, means defining an opening therein corresponding to the sample chamber and means defining a open area surrounding the opening, wherein the opening and open area form a separating wall of thin film therebetween; and   a transparent cover having first main surface superposable on the thin film to cover the at least one sample chamber when a sample is received therein, wherein the cover has a second main surface opposite the first main surface and having a thin film pattern thereon demarcating an area for aligning the area of the cover with the at least one sampling chamber.   
     
     
       11. The biological assembly according to claim 10, wherein the opening is circular. 
     
     
       12. The biological assembly according to claim 10, wherein the thin film has a height of from 10 to 50 μm. 
     
     
       13. The biological assembly according to claim 10, wherein the separating wall has a width of 200 to 800 μm. 
     
     
       14. The biological assembly according to claim 10, wherein said at least one sample chamber has a volume of 2 to 15 μl. 
     
     
       15. The biological assembly according to claim 10, wherein the thin film pattern is identical in configuration to said separating wall. 
     
     
       16. The biological assembly according to claim 10, wherein the cover has a grid pattern in the first main surface within the area demarcated by said thin film pattern. 
     
     
       17. The biological assembly according to claim 10, wherein the thin film comprises at least one of a photoresist, an epoxy and a hydrophobic ink.