Bispecific antibody determinants

Abstract

A homogenous sample of identical bispecific antibody determinants, each determinant being composed of two L-H half-molecules linked by disulfide bonds, each L-H half-molecule being specific for a different antigenic determinant and including at least the F(ab') portion of a monoclonal IgG antibody. The bispecific antibody determinants are useful, e.g., in the formation of multilamellar assemblies and in enzymatic assays.

What is claimed is: 
     
       1. A process for preparing covalent hybrid antibodies comprising the steps of: A) selectively cleaving a first monoclonal immunoglobulin molecule which is an antibody to a first antigen into its heavy chain-light chain half-molecules by cleavage of the inter-heavy chain disulfide linkage to produce half-molecules;   B) selectively cleaving a second monoclonal immunoglobulin molecule which is an antibody to the first or a second antigen into its heavy chain-light chain half-molecules by reduction of the inter-heavy chain disulfide linkage to produce reduced half-molecules; and   C) combining the half-molecules from step (A) with the reduced half-mulecules from step (B).