US5064758AExpiredUtility

Method of preparing a mixture of ribonucleotides

Assignee: INST MOREOLOGII CHELOVEKAPriority: Jun 14, 1988Filed: Dec 20, 1988Granted: Nov 12, 1991

Est. expiryJun 14, 2008(expired)· nominal 20-yr term from priority

Inventors:Boris B. Fux Marina E. Shabanova Svyatoslav N. Fedorov Jury M. Krasnopolsky Uldis Y. Mixtais Evgeny D. Ermolaev Mara A. Gailuma

A61P 27/02C12P 19/30

PatentIndex Score

Cited by

References

Claims

Abstract

PCT No. PCT/SU88/00269 Sec. 371 Date Feb. 1, 1990 Sec. 102(e) Date Feb. 1, 1990 PCT Filed Dec. 20, 1988 PCT Pub. No. WO89/12689 PCT Pub. Date Dec. 28, 1989.A method of preparing a mixture of ribonucleotides consisting in hydrolysis of yeast nucleic acid with pancreatic ribonuclease at a pH 4.5-5.5. Thereafter, separating the ribonucleotide fraction from the obtained hydrolyzate is effective on membranes with pores sized 50-150 ANGSTROM with subsequent purification and isolation of the end product.

Claims

exact text as granted — not AI-modified

We claim: 
     
       1. A method for preparing a ribonucleotide mixture comprising hydrolysis of yeast ribonucleic acid with pancreatic ribonuclease, separation of the ribonucleotide fraction from the obtained hydrolyzate with subsequent isolation of the end product, wherein the hydrolysis of the yeast ribonucleic is carried out at pH 4.5 to 5.5 and the separation of the ribonucleotide fraction from hydrolysate is effected on membranes with pores sized 50 to 150 Å. 
     
     
       2. A method according to claim 1, wherein the hydrolysis is carried out at 62°-65° C. 
     
     
       3. A method according to claims 1-2, wherein before separation of the ribonucleotide fraction, ethanol is added to the hydrolyzate in the amount of 15-30 per cent of the hydrolyzate volume. 
     
     
       4. A method according to claim 1, wherein the ribonucleotide fraction is separated on membranes at a temperature of 20°-30° C. and a pressure of 0.15-0.4 MPa. 
     
     
       5. A method according to claim 1, wherein the end product is isolated by precipitating the ribonucleotide fraction with 8-10 volumes of ethanol with subsequent removal of the solvent and drying of the end product. 
     
     
       6. A method according to claim 1, wherein the end product of the method is isolated by precipitating the ribonucleotide fraction with 8-10 volumes of ethanol with subsequent removal of the solvent, dissolution of the mixture in 0.55-0.65 per cent sodium chloride solution to the concentration of 3.3-3.7 per cent by weight, and filtration of the obtained solution through membranes with pores sized 2000-2200 Å under a pressure of 78-98 kPa.

Join the waitlist — get patent alerts

Track US5064758A — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.