US4953962AExpiredUtility

Microscope objective for accommodating different cover-glass thicknesses

Assignee: ZEISS STIFTUNGPriority: Apr 16, 1988Filed: Mar 30, 1989Granted: Sep 4, 1990
Est. expiryApr 16, 2008(expired)· nominal 20-yr term from priority
G02B 21/02G02B 27/0068
65
PatentIndex Score
31
Cited by
8
References
17
Claims

Abstract

A microscope objective adjustable for different thicknesses of cover-glass includes a second lens (L2 and L3) movable between a first lens (L1) and a third lens (L4) and a fourth lens (L5) movable with the second lens, but in an opposite direction. A ring that accomplishes movements of the second and fourth lenses also resets the focus of the objective. All movements are linear and are arranged to maintain the correction of image error while the objective is adjustable over a wide range of cover-glass thicknesses from 0 to 2 mm.

Claims

exact text as granted — not AI-modified
We claim: 
     
       1. In a microscope for observing objects covered by cover-glasses of differing thicknesses, an objective having a linear magnification of between 32× and 60×, an aperture of at least 0.5, and an optical axis along which are aligned, in the order of light transmission, a stationary first lens (L1), a movable second lens (L2/L3), and a stationary third lens (L4), said microscope objective comprising: a. a movable fourth lens (L5) arranged beyond said third lens in said order of light transmission; and   b. an adjusting device, selectively positionable in accordance with the thickness of the cover-glass covering an object being observed, for moving said second and fourth lenses to adjust the objective to compensate for said cover-glass thickness, the movement of said fourth lens being in a direction opposite to movement of said second lens.   
     
     
       2. The microscope objective of claim 1 wherein the movements of said second and fourth lenses are linear. 
     
     
       3. The microscope objective of claim 1 wherein the stroke of movement of said fourth lens is larger than the stroke of movement of said second lens. 
     
     
       4. The microscope objective of claim 3 wherein said strokes of movement differ by at least a factor of three. 
     
     
       5. The microscope objective of claim 1 wherein said third lens is held in a centering mount. 
     
     
       6. The microscope objective of claim 1 wherein all of said lenses are mounted within a carrier (11) which is itself movable along said optical axis for focusing the objective, and wherein the selective positioning of said adjusting device also moves said carrier for resetting the focus of said microscope objective. 
     
     
       7. The microscope objective of claim 6 wherein said adjusting device includes a single actuating ring arranged for causing said opposing movement of said second and fourth lenses and for moving said carrier. 
     
     
       8. The microscope objective of claim 7 wherein said adjusting device includes a thread on an intermediate ring coupled with said actuating ring. 
     
     
       9. The microscope objective of claim 8 wherein said adjusting device includes two cams connected respectively to mounts for said second and fourth lenses and engaged respectively in grooves of constant but different pitch in said intermediate ring. 
     
     
       10. The microscope objective of claim 1 wherein movement of said fourth lens to change an air separation distance (d10) from said stationary third lens changes a field correction of said microscope objective for astigmatism. 
     
     
       11. The microscope objective of claim 1 wherein both of said first and third lenses are positive single lenses. 
     
     
       12. The microscope objective of claim 1 wherein said movable second lens has a positive refractive power. 
     
     
       13. The microscope objective of claim 1 wherein said movable fourth lens is a cemented component of negative refractive power. 
     
     
       14. The microscope objective of claim 1 in a planachromatic form containing eight lenses in four groups, arranged so that: said first lens (L1) is in the form of a concave-convex converging lens;   said second lens includes a concave-convex single lens (L2) at a distance (d2) from said first lens (L1) and a cemented component (L3) consisting of three lenses, the middle lens of which is a bi-convex converging lens opposed by a convex-concave dispersing lens and a concave-convex dispersing lens;   said third lens (L4) is a bi-convex single lens at a distance (d8) from said cemented component (L3);   said fourth lens (L5) is a cemented component consisting of a bi-convex converging lens and a bi-concave dispersing lens at a distance (d10) from said third lens (L4); and said distances (d2, d8, and d10) are variable for said adjustment to said different cover-glass thicknesses.     
     
     
       15. The microscope objective of claim 14 wherein said lenses are structured according to the following table for lens radii r, lens thicknesses and distances d, indexes of refraction nd, and Abbe numbers γd of the values of said lenses, to produce the indicated linear magnification and numerical aperture and accommodate cover-glass thicknesses from zero to 2 millimeters, as follows:                                     TABLE I                                 
__________________________________________________________________________
                           Index of                                       
                                Abbe                                      
   Radius  Thickness                                                      
                 Distance  Refrac-                                        
                                Number                                    
Lens                                                                      
   r/mm    d/mm  d/mm      tion nd                                        
                                γ d                                 
__________________________________________________________________________
    r1 = -3.566                                                           
L1          d1 = 3.24      1.5205                                         
                                69.7                                      
    r2 = -3.839                                                           
                  d2 = 1.43 to 0.48                                       
    r3 = -659.4                                                           
L2          d3 = 2.50      1.5891                                         
                                61.3                                      
    r4 = -10.96                                                           
                  d4 = 0.62                                               
    r5 = 48.448                                                           
            d5 = 1.0       1.6134                                         
                                44.3                                      
    r6 = 7.515                                                            
L3          d6 = 4.7       1.4866                                         
                                84.5                                      
    r7 = -8.485                                                           
            d7 = 1.0       1.8807                                         
                                41.0                                      
    r8 = -24.36                                                           
                  d8 = 0.20 to 1.15                                       
    r9 = 17.013                                                           
L4          d9 = 3.0       1.5285                                         
                                77.0                                      
   r10 = -25.925                                                          
                 d10 = 15.75 to 19.06                                     
   r11 = 8.593                                                            
           d11 = 4.0       1.4585                                         
                                67.7                                      
L5 r12 = -29.03                                                           
           d12 = 1.5       1.6967                                         
                                56.4                                      
   r13 = 5.975                                                            
__________________________________________________________________________
 Cover-glass: 0 to 2 mm                                                   
 Working distance: W = 3.3 mm to 2.2 mm                                   
 Linear magnification: β = 40 x                                      
 Numerical aperture: A = 0.6.                                             
     
     
     
       16. The microscope objective of claim 14 wherein said lenses are structured according to the following table for lens radii r, lens thicknesses and distances d, indexes of refraction nd, and Abbe numbers γd of the values of said lenses, to produce the indicated linear magnification and numerical aperture and accommodate cover-glass thicknesses from zero to 2 millimeters, as follows:                                     TABLE II                                
__________________________________________________________________________
                           Index of                                       
                                Abbe                                      
   Radius  Thickness                                                      
                 Distance  Refrac-                                        
                                Number                                    
Lens                                                                      
   r/mm    d/mm  d/mm      tion nd                                        
                                γ d                                 
__________________________________________________________________________
    r1 = -2.751                                                           
L1          d1 = 2.05      1.5205                                         
                                69.7                                      
    r2 = -3.110                                                           
                  d2 = 1.44 to 0.21                                       
    r3 = -132.1                                                           
L2          d3 = 2.15      1.4866                                         
                                84.5                                      
    r4 = -7.032                                                           
                  d4 = 3.38                                               
    r5 = 34.383                                                           
            d5 = 2.0       1.5986                                         
                                46.9                                      
    r6 = 9.040                                                            
L3          d6 = 5.0       1.4866                                         
                                84.5                                      
    r7 = -7.766                                                           
            d7 = 1.0       1.8807                                         
                                41.0                                      
    r8 = -17.974                                                          
                  d8 = 3.73 to 4.96                                       
    r9 = 15.281                                                           
L4          d9 = 3.0       1.4866                                         
                                84.5                                      
   r10 = -163.8                                                           
                 d10 = 10.48 to 16.07                                     
   r11 = 5.862                                                            
           d11 = 3.9       1.4875                                         
                                70.4                                      
L5 r12 = -21.964                                                          
           d12 = 1.0       1.6228                                         
                                56.9                                      
   r13 = 4.278                                                            
__________________________________________________________________________
 Cover-glass: 0 to 2 mm                                                   
 Working distance: W = 2.4 mm to 1.55 mm                                  
 Linear magnification: β = 32 x                                      
 Numerical aperture: A = 0.55.                                            
     
     
     
       17. The microscope objective of claim 14 wherein said lenses are structured according to the following table for lens radii r, lens thicknesses and distances d, indexes of refraction nd, and Abbe numbers γd of the values of said lenses, to produce the indicated linear magnification and numerical aperture and accommodate cover-glass thicknesses from zero to 2 millimeters, as follows:                                     TABLE III                               
__________________________________________________________________________
                           Index of                                       
                                Abbe                                      
   Radius  Thickness                                                      
                 Distance  Refrac-                                        
                                Number                                    
Lens                                                                      
   r/mm    d/mm  d/mm      tion nd                                        
                                γ d                                 
__________________________________________________________________________
    r1 = -3.563                                                           
L1          d1 = 2.21      1.5205                                         
                                69.7                                      
    r2 = -3.091                                                           
                  d2 = 1.40 to 0.11                                       
    r3 = -354.6                                                           
L2          d3 = 2.22      1.4866                                         
                                84.5                                      
    r4 = -8.262                                                           
                  d4 = 0.9                                                
    r5 = 39.776                                                           
            d5 = 0.9       1.6134                                         
                                44.3                                      
    r6 = 8.778                                                            
L3          d6 = 4.63      1.4866                                         
                                84.5                                      
    r7 = -6.945                                                           
            d7 = 1.02      1.8807                                         
                                41.0                                      
    r8 = -17.314                                                          
                  d8 = 0.19 to 1.48                                       
    r9 = 13.598                                                           
L4          d9 = 3.4       1.4866                                         
                                84.5                                      
   r10 = -54.136                                                          
                 d10 = 13.85 to 20.30                                     
   r11 = 5.143                                                            
           d11 = 3.87      1.4585                                         
                                67.7                                      
L5 r12 = -10.086                                                          
           d12 = 1.24      1.6511                                         
                                55.6                                      
   r13 = 3.927                                                            
__________________________________________________________________________
 Cover-glass: 0 to 2 mm                                                   
 Working distance: W = 2.35 mm to 1.3 mm                                  
 Linear magnification: β = 50 x                                      
 Numerical aperture: A = 0.65.

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