Method for preparing dense nutrient medium for culturing microorganisms

Abstract

A method for preparing a dense nutrient medium for culturing microorganisms comprises copolymerization of acrylamide, N,N'-methylenebisacrylamide and a branched polyacrylamide with the mean molecular mass of 7.2×10 4 at a mass ratio therebetween of 7.0-15:0.11-0.21:0.028-0.11 respectively and of an initiator in a physiological solution till the formation of a gel which is then washed and impregnated with a nutrient substrate.

We claim: 
     
       1. A method for preparing a dense nutrient medium for culturing micoorganisms comprising copolymerization of acrylamide and N,N'-methylenebisacrylamide in the presence of an initiator in a physiological solution till the formation of a gel, washing and impregnation thereof with a nutrient substrate, characterized in that said copolymerization of acrylamide and N,N'-methylenebisacrylamide is conducted in the presence of a branched polyacrylamide with the mean molecular mass of 7.2×10 4  at a mass ratio thereof equal to 7.0-15:0.11:-0.21:0.028-0.11 respectively. 
     
     
       2. A method according to claim 1, characterized in that an aqueous solution of said polyacrylamide is used. 
     
     
       3. A method according to claims 1 and 2, characterized in that said impregnation of said gel is effected by means of a nutrient substrate containing polyethyleneoxide in an amount of from 0.02 to 0.2 g/l.