Deacetylated polysaccharide S-60

Abstract

Deacetylated Polysaccharide S-60, prepared by deacetylating the polysaccharide S-60 produced by fermentation of Pseudomonas elodea, ATCC 31461, has valuable properties in both the clarified and non-clarified form, and is useful as an agar substitute or a shapeable room deodorant. The non-clarified deacetylated gum contains about 17% protein and principally carbohydrate, which comprises glucuronic acid ( DIFFERENCE 13% based on wt. gum) and the neutral sugars rhamnose and glucose in the approximate molar ratio 3:2. The clarified, deacetylated gum contains mostly carbohydrate and no more than about 2% protein.

What is claimed is: 
     
       1. A microbiological culture medium which comprises a nutrient and about 0.5-5% deacetylated heteropolysaccharide selected from the group consisting of heteropolysaccharide S-60 which comprises (a) about 50% (wt./wt.) insoluble material of which about 34% (wt.wt.) is protein and (b) about 50% (wt./wt.) carbohydrate which contains about 22-26% (wt./wt.) glucuronic acid, 0% acetyl groups, and the neutral sugars rhamnose and glucose in the approximate molar ratio 3:2, said rhamnose and glucose sugars being primarily 1,4 β-linked, and clarified heteropolysaccharide S-60, which comprises no more than about 2% (wt./wt.) protein and carbohydrate, said carbohydrate containing about 22-26% (wt./wt.) glucuronic acid, 0% acetyl groups, and the neutral sugars rhamnose and glucose in the approximate molar ratio 3:2, said rhamnose and glucose sugars being primarily 1,4 β-linked, said heteropolysaccharide being further characterized in that it is anionic, and forms brittle, thermoreversible gels. 
     
     
       2. A medium of claim 1 which comprises about 1-2% heteropolysaccharide.