Carrier-based agricultural biofertilizer composition

Abstract

Exemplary embodiments of the present disclosure are directed towards the carrier-based agricultural biofertilizer composition, includes an isolated Bacillus Cereus strain CGAPGPBBS-048 having the deposit accession number KY495213; an agriculturally acceptable liquid formulation including a stabilizer, that may provide stability and support for at least one of the Bacillus species, thereby enhancing the growth and overall health of the plants.

We claim: 
     
         1 . A carrier-based agricultural biofertilizer composition, comprising:
 an isolated  Bacillus Cereus  strain CGAPGPBBS-048 having the deposit accession number KY495213;   an agriculturally acceptable liquid formulation including a stabilizer, whereby the stabilizer provides stability and support for at least one of the  Bacillus  species, thereby enhancing the growth and overall health of the plants.   
     
     
         2 . The composition, as claimed in  claim 1 , wherein the  Bacillus Cereus  strain CGAPGPBBS-048 is capable of producing indole acetic acid in higher pH conditions by expressing a gene encoding an indole acetic acid-producing enzyme. 
     
     
         3 . The composition as claimed in  claim 1 , wherein the  Bacillus Cereus  strain CGAPGPBBS-048 was isolated from rhizosphere soil of Yola, Nigeria. 
     
     
         4 . The composition as claimed in  claim 1 , wherein the  Bacillus Cereus  strain CGAPGPBBS-048 was isolated by a serial dilution technique. 
     
     
         5 . The composition as claimed in  claim 1 , the  Bacillus Cereus  strain CGAPGPBBS-048 has the ability to stimulate plant growth through the production of phytohormones, including indoleacetic acid, thereby regulating plant growth, improving root development, and enhancing overall plant vigor. 
     
     
         6 . A method for enhancing soil fertility using a carrier-based agricultural biofertilizer composition, comprising the steps of:
 preparing a nutrient medium containing optimized sources of carbon, nitrogen, and phosphate, conducive to bacteria growth;   inoculating the nutrient medium with a pure culture of a  Bacillus Cereus  strain CGAPGPBBS-048, characterized by deposit accession number KY495213;   fermenting the inoculated medium under precisely controlled conditions until the broth attains a concentration of 3.0×10{circumflex over ( )}9 CFU/mL;   introducing polyvinyl pyrrolidone (PVP) into the broth at a concentration of 0.5%;   producing a bacterial formulation by culminating the fermentation process;   diluting the bacterial formulation with precision to attain a targeted microbial concentration of 3×10{circumflex over ( )}8 CFU/mL; and   systematically applying the meticulously prepared bacterial formulation to the soil using appropriate methods to ensure even and comprehensive distribution.   
     
     
         7 . The method as claimed in  claim 6 , wherein the nutrient medium comprises carbon sources selected from the group consisting of sucrose and glucose, whereby the carbon sources provide optimal conditions for bacterial growth and nutrient assimilation. 
     
     
         8 . The method as claimed in  claim 6 , wherein the nutrient medium comprises nitrogen sources selected from the group consisting of ammonium sulfate, urea, and peptone, whereby the nitrogen sources provide optimal conditions for bacterial growth and nutrient assimilation. 
     
     
         9 . The method as claimed in  claim 6 , wherein the step of fermenting conditions is carried out under controlled conditions including temperature, pH, and oxygen levels, to thereby promote optimal bacterial growth and metabolic activity. 
     
     
         10 . The method as claimed in  claim 6 , wherein the step of systematically applying the meticulously prepared bacterial formulation to soil is performed using sprinkler irrigation, spray application, drone-based application, soil mixing techniques, seed deepening methods, plant root deepening methods, or any combination thereof.