Extracellular matrix-containing composition and method for producing same, and three-dimensional tissue construct and method for producing same

Abstract

The present invention relates to an extracellular matrix-containing composition containing a fragmented extracellular matrix component and a compound bound or adsorbed to the fragmented extracellular matrix component.

1 . A method for producing an extracellular matrix-containing composition, comprising
 a process of bringing a fragmented extracellular matrix component into contact with a compound that is able to be bound or adsorbed to the fragmented extracellular matrix component.   
     
     
         2 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein the fragmented extracellular matrix component is obtained by fragmenting an extracellular matrix component in an aqueous medium.   
     
     
         3 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein the fragmented extracellular matrix component contains a fragmented collagen component.   
     
     
         4 . A method for producing a three-dimensional tissue construct, comprising:
 a first process in which a extracellular matrix-containing composition is brought into contact with cells in an aqueous medium; and   a second process in which the cells in contact with the extracellular matrix-containing composition are cultured,   wherein the extracellular matrix-containing composition, comprises a fragmented extracellular matrix component and a compound bound or adsorbed to the fragmented extracellular matrix component.   
     
     
         5 . The method for producing a three-dimensional tissue construct according to  claim 4 , comprising
 a process in which, in the aqueous medium, the fragmented extracellular matrix component, a compound bound or adsorbed to the fragmented extracellular matrix component, and the cells are precipitated, after the first process and before the second process.   
     
     
         6 . The method for producing a three-dimensional tissue construct according to  claim 4 ,
 wherein the first process is performed after a layer of the cells is formed in the aqueous medium.   
     
     
         7 . The method for producing a three-dimensional tissue construct according to  claim 4 ,
 wherein the cells contain extracellular matrix producing cells.   
     
     
         8 . The method for producing a three-dimensional tissue construct according to  claim 4 ,
 wherein the cells are one or more types of cells selected from a group consisting of vascular endothelial cells, cancer cells, cardiomyocytes, smooth muscle cells, and epithelial cells.   
     
     
         9 . The method for producing a three-dimensional tissue construct according to  claim 4 ,
 wherein a total content of the fragmented extracellular matrix component and the compound based on a total mass of the fragmented extracellular matrix component, the compound and the cells is 10 mass % or more and 90 mass % or less.   
     
     
         10 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein an average length of the fragmented extracellular matrix component is 100 nm or more and 100 μm or less.   
     
     
         11 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein an average diameter of the fragmented extracellular matrix component is 50 nm to 30 μm.   
     
     
         12 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein the fragmented extracellular matrix component maintains a triple helix structure derived from collagen.   
     
     
         13 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein the fragmented extracellular matrix component is fragmented by a homogenizer in an aqueous media.   
     
     
         14 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein an entire surface or a part thereof of the fragmented extracellular matrix component is coated with the compound.   
     
     
         15 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein a content of the compound with respect to 100 parts by mass of the fragmented extracellular matrix component is 0.5 parts by mass or more and 10 parts by mass or less.   
     
     
         16 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein a form of the extracellular matrix-containing composition is a solid or powder form.   
     
     
         17 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein an average length of the fragmented extracellular matrix component is 5 μm or more and 30 μm or less,   the compound is at least one selected from a group consisting of heparan sulfate, chondroitin sulfate, and fibronectin, and   a content of the compound with respect to 100 parts by mass of the fragmented extracellular matrix component is 0.5 parts by mass or more and 10 parts by mass or less.   
     
     
         18 . The method for producing an extracellular matrix-containing composition according to  claim 1 ,
 wherein the fragmented extracellular matrix component is fragmented by a homogenizer in an aqueous media, and   the compound is at least one selected from a group consisting of heparan sulfate, chondroitin sulfate, and fibronectin.