Reagent for detecting methyl salicylate, methyl salicylate sensor, method for sensing methyl salicylate using the same and methods for detecting plant pathogen infections

Abstract

The present disclosure provides a reagent for detecting methyl salicylate, which is a plant hormone released when a plant is infected with a disease, in the cultivation of plants including agricultural crops, a methyl salicylate sensor, and a method for sensing methyl salicylate, using the same, thereby providing a method for detection of pathogen infection in a plant. A reagent for detecting methyl salicylate, comprising a terbium compound doped with a rare earth element other than terbium. A methyl salicylate sensor comprising a capture section of methyl salicylate having the reagent and a detection section detecting that methyl salicylate has been captured by the capture section.

1 . A reagent for detecting methyl salicylate, comprising a terbium compound doped with rare earth element other than terbium. 
     
     
         2 . The reagent according to  claim 1 , wherein the terbium compound doped with rare earth element other than terbium is represented by the following general formula (1):
   Ln c TbX a Y b   (1)
   
       (wherein Ln represents at least one rare earth element other than terbium, X and Y each independently represent at least one selected from the group consisting of acetate ion, propionate ion, butyrate ion, pivalate ion, 2-methylbutyrate ion, trifluoroacetate ion, benzoate ion, chloride ion, and nitrate ion, c represents a number ranging from 0.015 to 0.05, and a and b each independently represent a number that satisfies 0≤a≤3.15, 0≤b≤3.15, and a+b=3+3×c). 
     
     
         3 . The reagent according to  claim 1 , wherein the rare earth element other than terbium is at least one selected from the group consisting of scandium, yttrium, lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, dysprosium, holmium, erbium, thulium, ytterbium, and lutetium. 
     
     
         4 . The reagent according to  claim 1 , further comprising a non-volatile ionic liquid. 
     
     
         5 . The reagent according to  claim 4 , wherein the non-volatile ionic liquid is at least one selected from the group consisting of 1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium acetate, 1-ethyl-3-methylimidazolium tetrafluoroborate, 1-butyl-1-methylpyrrolidinium bis(fluorosulfonyl)imide, 1-butylpyridinium bis(trifluoromethanesulfonyl)imide, 1-methyl-1-propylpiperidinium bis(fluorosulfonyl)imide, 1-ethyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide, and 1-butyl-3-methylimidazolium methyl dicyanamate. 
     
     
         6 . A methyl salicylate sensor for detecting methyl salicylate, comprising:
 a capture section of methyl salicylate having a reagent comprising a terbium compound doped with rare earth element other than terbium, and   a detection section detecting that methyl salicylate has been captured by the capture section.   
     
     
         7 . The methyl salicylate sensor according to  claim 6 , wherein the capture section comprises a medium containing the reagent. 
     
     
         8 . The methyl salicylate sensor according to  claim 7 , wherein the medium is paper, glass fiber, resin, or water-soluble polymer. 
     
     
         9 . A method for sensing methyl salicylate, comprising detecting methyl salicylate using the reagent according to  claim 1 , comprising:
 (i) reacting a terbium compound doped with rare earth element other than terbium with methyl salicylate to form a complex;   (ii) exposing the complex to excitation light; and   (iii) detecting fluorescence emitted by the complex.   
     
     
         10 . A method for detecting pathogen infection in a plant by placing the reagent according to  claim 1  in the vicinity of a plant and confirming fluorescence emission derived from a complex formed by a reaction between a terbium compound doped with a rare earth element other than terbium and methyl salicylate. 
     
     
         11 . The method according to  claim 10 , wherein the vicinity of a plant is a distance within 2 m of the plant to be monitored. 
     
     
         12 . The reagent according to  claim 2 , wherein the rare earth element other than terbium is at least one selected from the group consisting of scandium, yttrium, lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, dysprosium, holmium, erbium, thulium, ytterbium, and lutetium. 
     
     
         13 . The reagent according to  claim 2 , further comprising a non-volatile ionic liquid. 
     
     
         14 . A method for sensing methyl salicylate, comprising detecting methyl salicylate using the reagent according to  claim 2 , comprising:
 (i) reacting a terbium compound doped with rare earth element other than terbium with methyl salicylate to form a complex;   (ii) exposing the complex to excitation light; and   (iii) detecting fluorescence emitted by the complex.   
     
     
         15 . A method for detecting pathogen infection in a plant by placing the reagent according to  claim 2  in the vicinity of a plant and confirming fluorescence emission derived from a complex formed by a reaction between a terbium compound doped with a rare earth element other than terbium and methyl salicylate. 
     
     
         16 . The method according to  claim 15 , wherein the vicinity of a plant is a distance within 2 m of the plant to be monitored.