Herbal bioactives based immunostimulant formulation for poultry birds & cattle and preparation thereof

Abstract

A stable and highly bioavailable herbal bioactives-based immuno-stimulant formulation can boost the immune system, combat infections, and enhance growth and performance in poultry birds and cattle. A method of preparing a stable and highly bioavailable herbal bioactives-based immuno-stimulant formulation includes converting the plant bioactive molecules to multi molecular colloids using an ultra-sonication process followed by the stabilization of said colloidal particles through steric interactions.

What is claimed is: 
     
         1 . An immuno-stimulant herbal formulation comprising plant bioactive molecules and an essential amino acid encapsulated in a polymer composite, wherein the plant bioactive molecules comprise curcumin, withanolides, thymol and eugenol or the plant bioactive molecules comprise curcumin, safed musli extract, thymol and oregano oil. 
     
     
         2 . The immuno-stimulant herbal formulation according to  claim 1 , wherein the amino acid is selected from glycine, lysine, glutamine, tryptophan, or mixture thereof. 
     
     
         3 . The immuno-stimulant herbal formulation according to  claim 1 , wherein the polymer composite is a chitosan and whey protein. 
     
     
         4 . The immuno-stimulant herbal formulation according to  claim 1 , wherein the curcumin is in the range of 2000 to 5000 ppm, the withanolides is in the range of 2000 to 3000 ppm, the thymol is in the range of 3000 to 5000 ppm and the eugenol is in the range of 2000 to 3000 ppm. 
     
     
         5 . The immuno-stimulant herbal formulation according to  claim 1 , wherein the amino acid is in the range of 1000 to 5000 ppm. 
     
     
         6 . The immuno-stimulant herbal formulation according to  claim 2 , wherein the amino acid comprises a mixture of glycine, lysine, glutamine and tryptophan in a ratio of 5:4:2:1. 
     
     
         7 . The immuno-stimulant herbal formulation according to  claim 1 , wherein the particle size of the plant bioactive molecules alone is about 10-50 nm. 
     
     
         8 . The immuno-stimulant herbal formulation according to  claim 1 , wherein the particle size of components of the herbal formulation is in the range of about 10-100 nm. 
     
     
         9 . A method of preparation of an immuno-stimulant herbal formulation comprising:
 (a) preparing an active ingredients blend encapsulated in polysorbate, wherein the active ingredients blend includes curcumin, withanolides, thymol and eugenol or the active ingredients blend includes curcumin, safed musli extract, thymol and oregano oil, the preparation comprises the steps of:
 i. dissolving the curcumin in the polysorbate with sonication; 
 ii. dissolving the withanolides or the safed musli extract in water; 
 iii. dissolving the thymol and the eugenol, or the thymol and the oregano oil, in a solvent; and 
 iv. adding a solution from step (ii) to a solution of step (i) followed by a solution from step (iii), and thoroughly homogenizing a resulting solution using high sheer homogenizer; 
   (b) preparing a polymer matrix using a chitosan and whey protein;   (c) preparing an amino acid mix of glycine, lysine, glutamine and tryptophan in water;   (d) adding the active ingredients blend encapsulated in polysorbate obtained in step (a) to the polymer matrix obtained in step (b);   (e) adding the amino acid mix obtained from step (c) to a solution of step (d) and homogenizing;   (f) subjecting a solution of step (e) to sonication to ensure homogenized particle distribution; and   (g) optionally, adding anti-microbial agents to the solution obtained in step (f) prior to bottling.   
     
     
         10 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein the step (a) (i) comprises dissolving the curcumin in a weight percentage range of 0.2 to 0.5% in the polysorbate. 
     
     
         11 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein the step (a) (ii) comprises dissolving the withanolides in a weight percentage range of 0.2 to 0.3% in water. 
     
     
         12 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein in the step (a) (iii), the solvent is selected from an alcohol, methanol, ethanol, isopropanol, an aryl alcohol, or a mixture thereof. 
     
     
         13 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein in the step (a) (iii), the weight percentage of the thymol and the eugenol is in a range of 0.3 to 0.5% and 0.2 to 0.3%, respectively, and the solvent is ethanol. 
     
     
         14 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein the step (b) comprises using 0.1% (w/v) chitosan and 0.05% (w/v) whey protein in a ratio of 2:1. 
     
     
         15 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein the step (c), the amino acid mix is a mixture of glycine, lysine, glutamine and tryptophan in a ratio of 5:4:2:1. 
     
     
         16 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein in the step (e), homogenizing is performed at 600-800 rpm. 
     
     
         17 . The method of preparation of the immuno-stimulant herbal formulation according to  claim 9 , wherein in the steps (a) and (f), the sonication is performed for 10-20 minutes. 
     
     
         18 . A method of treating poultry birds and cattle to enhance an immunity therein, the method comprising administering an effective amount of the immune-stimulant herbal formulation according to  claim 1 , to one or more of the poultry birds and the cattle. 
     
     
         19 . The method of treating poultry birds and cattle according to  claim 18 , wherein:
 the poultry bird is selected from chicken, turkey, duck, goose, guinea fowl, quail and pigeon; and   the cattle is selected from cow, ox, bull, calf, sheep and goat.