Tomato variety nun 00333 top

Abstract

A new and distinct tomato variety NUN 00333 TOP is disclosed, as well as seeds and plants and fruits thereof.

1 . A plant or seed of tomato variety NUN 00333 TOP, wherein a representative sample of seed of said tomato variety NUN 00333 TOP has been deposited under Accession Number NCIMB ______. 
     
     
         2 . A plant part of the plant of  claim 1 , wherein said plant part is a fruit, a leaf, a cell, a scion, a root, a rootstock, or a cutting. 
     
     
         3 . A seed that produces the plant of  claim 1 . 
     
     
         4 . A tomato plant or a part thereof having all the physiological and morphological characteristics of the plant of  claim 1  when grown under the same environmental conditions. 
     
     
         5 . A tissue culture or cell culture of regenerable cells of the plant or plant part of  claim 1 , wherein said cells are obtained from tomato variety NUN 00333 TOP and are suitable for regeneration into a plant having all of the physiological and morphological characteristics of tomato variety NUN 00333 TOP. 
     
     
         6 . A method of producing the plant of  claim 1  or a part thereof, said method comprising vegetative propagating of at least a part of the plant of tomato variety NUN 00333 TOP, wherein said part is a cutting, a cell culture, or a tissue culture, and wherein a representative sample of seed of said tomato variety NUN 00333 TOP has been deposited under Accession Number NCIMB ______. 
     
     
         7 . A vegetatively propagated plant, or a part thereof, produced by the method of  claim 6 , wherein the plant or a part thereof has all of the physiological and morphological characteristics of the plant of tomato variety NUN 00333 TOP when grown under the same environmental conditions, and wherein a representative sample of seed of said tomato variety NUN 00333 TOP has been deposited under Accession Number NCIMB ______. 
     
     
         8 . A method of producing a tomato plant, said method comprising crossing the plant of  claim 1  with a second tomato plant at least once, producing a progeny tomato plant from said crossing, and optionally allowing the progeny tomato plant to form seed. 
     
     
         9 . A method of making doubled haploids of the plant of  claim 1 , said method comprising making double haploid cells from haploid cells of the plant or plant part of tomato variety NUN 00333 TOP, wherein a representative sample of seed of said tomato variety NUN 00333 TOP has been deposited under Accession Number NCIMB ______. 
     
     
         10 . A plant comprising the scion or rootstock of  claim 2 . 
     
     
         11 . A container comprising the plant or seed of  claim 1 . 
     
     
         12 . A food, a feed, or a processed product comprising the plant part of  claim 2 , wherein the plant part comprises at least a regenerable cell of tomato variety NUN 00333 TOP. 
     
     
         13 . A method of introducing a desired trait into the plant of  claim 1 , said method comprises transforming the plant of  claim 1  with a transgene that confers the desired trait, wherein the transformed plant otherwise comprises the desired trait and otherwise has all the physiological and morphological characteristics of tomato variety NUN 00333 TOP, and wherein the desired trait is male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism. 
     
     
         14 . A method of producing a tomato fruit, said method comprising growing the plant of  claim 1  until it sets at least one fruit, and collecting the fruit. 
     
     
         15 . A fruit produced by the method of  claim 14 . 
     
     
         16 . A method of producing a modified tomato plant having a desired trait, said method comprises mutating a plant or plant part of tomato variety NUN 00333 TOP and selecting the plant with a desired trait, wherein a representative sample of seed of said tomato variety has been deposited under Accession Number NCIMB ______, wherein the modified tomato plant has the desired trait and otherwise has all of the physiological and morphological characteristics of tomato variety NUN 00333 TOP, and wherein the desired trait is male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism. 
     
     
         17 . A method of producing a tomato seed, said method comprises crossing tomato plants and harvesting the resultant seed, wherein at least one tomato plant in the cross is the plant of  claim 1 , wherein a representative sample of seed of said tomato variety NUN 00333 TOP has been deposited under Accession Number NCIMB ______. 
     
     
         18 . A method of introducing a single locus conversion into the plant of  claim 1 , comprising:
 a. crossing the plant of  claim 1  with a second tomato plant comprising a desired single locus conversion to produce F1 progeny plants;   b. selecting the F1 progeny plants that have the single locus conversion to produce selected F1 progeny plants;   c. crossing selected F1 progeny plants with tomato variety NUN 00333 TOP to produce backcross progeny plants;   d. selecting backcross progeny plants that have the single locus conversion and otherwise comprise all of the physiological and morphological characteristics of tomato variety NUN 00333 TOP to produce selected backcross progeny plants; and   e. repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the single locus conversion and otherwise comprise all of the physiological and morphological characteristics of tomato variety NUN 00333 TOP, wherein a representative sample of seed of said tomato variety NUN 00333 TOP has been deposited under Accession Number NCIMB ______.   
     
     
         19 . A tomato plant produced by the method of  claim 18 , wherein the plant comprises the single locus conversion and otherwise has all the physiological and morphological characteristics of tomato variety NUN 00333 TOP, wherein the single locus conversion confers male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism. 
     
     
         20 . A method of producing a tomato plant derived from the plant of  claim 1 , comprising:
 a. producing a progeny tomato plant from tomato variety NUN 00333 TOP by crossing the plant of  claim 1  with itself or with a second tomato plant;   b. crossing the progeny plant with itself or a different tomato plant to produce a seed of a progeny plant of a subsequent generation;   c. growing a progeny plant of the subsequent generation from said seed and crossing the progeny plant of the subsequent generation with itself or another tomato plant; and   d. repeating steps (b) and (c) for a least one more generation to produce a tomato plant derived from tomato variety NUN 00333 TOP.   
     
     
         21 . A method of determining the genotype of the plant of  claim 1 , said method comprises obtaining a sample of nucleic acids from said plant, detecting in said nucleic acids a plurality of polymorphisms, thereby determining the genotype of the plant, and storing the results of detecting the plurality of polymorphisms on a computer readable medium. 
     
     
         22 . A method of developing a tomato plant in a tomato breeding program, said method comprises applying plant breeding techniques to the plant of  claim 1  or part thereof. 
     
     
         23 . A method of producing a modified tomato plant, said method comprising mutating a target gene by targeted gene editing in tomato plant or a plant part of tomato variety NUN 00333 TOP, wherein said target gene modifies a desired trait and wherein the desired trait is male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism. 
     
     
         24 . A tomato plant produced by the method of  claim 23 .