Culture Structure, Culture Method and Culture Chip

Abstract

A culture structure, a culture method and a culture chip are provided. The culture structure includes a culture plate and a vibration structure provided on the culture plate; the culture plate includes a plurality of accommodating structures configured to accommodate culture solution; the vibration structure includes a vibration signal generating structure and a plurality of vibration members; the vibration signal generating structure is configured to generate a vibration signal; the plurality of vibration members are connected to the vibration signal generating structure and configured to drive the culture solution in the plurality of accommodating structures to move according to the vibration signal.

1 . A culture structure, comprising a culture plate and a vibration structure provided on the culture plate; wherein
 the culture plate comprises a plurality of accommodating structures configured to accommodate culture solution;   the vibration structure comprises a vibration signal generating structure and a plurality of vibration members;   the vibration signal generating structure is configured to generate a vibration signal; and   the plurality of vibration members are connected to the vibration signal generating structure and configured to drive the culture solution in the plurality of accommodating structures to move according to the vibration signal.   
     
     
         2 . The culture structure according to  claim 1 , wherein the vibration structure further comprises a cover plate;
 an accommodating structure comprises an opening, and the opening of the accommodating structure is arranged to face a side of the cover plate;   the cover plate is provided on a side of the culture plate located at the opening of the accommodating structure;   the plurality of vibration members are provided on a side of the cover plate facing the culture plate, any one of the vibration members is arranged corresponding to one of the accommodating structures; a vibration member comprises a first end and a second end which are arranged opposite to each other, the first end of the vibration member is connected to the cover plate, and the second end of the vibration member extends away from the cover plate into the culture solution of a corresponding accommodating structure; and   the vibration signal generating structure is provided on the cover plate, connected to the plurality of vibration members through the cover plate, and applies the vibration signal to the vibration members through the cover plate.   
     
     
         3 . The culture structure according to  claim 2 , wherein the vibration signal generating structure comprises at least two piezoelectric transducers, the at least two piezoelectric transducers are arranged at an edge of the cover plate, and propagation directions of vibration signals generated by the at least two piezoelectric transducers intersect with each other in a plane where the cover plate is located. 
     
     
         4 . The culture structure according to  claim 3 , wherein the vibration signal generating structure comprises two piezoelectric transducers, and an angle between propagation directions of vibration signals generated by the two piezoelectric transducers is 80° to 100° in the plane where the cover plate is located. 
     
     
         5 . The culture structure according to  claim 3 , wherein the cover plate is a rectangular structure, the two piezoelectric transducers are provided on two sides of the cover plate that are perpendicular to each other, and the propagation directions of the vibration signals generated by the two piezoelectric transducers are orthogonal in the plane where the cover plate is located. 
     
     
         6 . The culture structure according to  claim 2 , wherein the vibration signal generating structure comprises a plurality of piezoelectric transducers, the plurality of piezoelectric transducers correspond to the plurality of vibration members respectively, and a vibration signal generated by each piezoelectric transducer is applied to a corresponding vibration member,
 wherein the piezoelectric transducer is an annular piezoelectric transducer; and   an orthographic projection of the piezoelectric transducer on the cover plate is at least partially overlapped with an orthographic projection of the corresponding vibration member on the cover plate.   
     
     
         7 . The culture structure according to  claim 2 , wherein a depth of the culture solution is 1 mm to 4 mm, a depth of the vibration member extending to the culture solution of the corresponding accommodating structure is 0.5 mm to 3 mm, and a distance between the vibration member and a bottom of the accommodating structure is 0.5 mm to 1.5 mm. 
     
     
         8 . The culture structure according to  claim 2 , wherein orthographic projections of the plurality of vibration members on the culture plate are located within a range of orthographic projections of the plurality of accommodating structures on the culture plate,
 wherein an area of an orthographic projection of the vibration member on the culture plate is 30% to 70% of an area of an orthographic projection of an accommodating structure corresponding to the vibration member on the culture plate.   
     
     
         9 . (canceled) 
     
     
         10 . The culture structure according to  claim 4 , wherein the vibration member is a vertebral structure, and an area of an orthographic projection of a first end of the vibration member on the cover plate is larger than an area of an orthographic projection of a second end of the vibration member on the cover plate,
 wherein end faces of the first end and the second end of the vibration member are both circular, a diameter of an end face of the first end of the vibration member is 0.4 mm to 0.8 mm, a diameter of an end face of the second end of the vibration member is 0.1 mm to 0.3 mm, and the accommodating structure is a hollow cylindrical structure with an inner diameter of 14 mm to 18 mm.   
     
     
         11 . (canceled) 
     
     
         12 . The culture structure according to  claim 4 , wherein the vibration member is a column structure, and end faces of a first end and a second end of the column structure are both circular,
 wherein diameters of the end faces of the first end and the second end of the column structure are 0.1 mm to 6 mm, the accommodating structure is a hollow cylindrical structure or a hollow cuboid structure, an inner diameter of the hollow cylindrical structure is 0.5 mm to 26 mm, an opening position of the accommodating structure of the hollow cuboid structure is square, a side length of the square opening position is 0.5 mm to 26 mm, and a plane where the opening position is located is parallel to a plane where the cover plate is located; and   an orthographic projection of the column structure on the cover plate falls within a range of an orthographic projection of the accommodating structure on the cover plate.   
     
     
         13 . (canceled) 
     
     
         14 . The culture structure according to  claim 12 , wherein the diameters of the end faces of the first end and the second end of the column structure are 0.1 mm to 0.4 mm; and
 the inner diameter of the hollow cylindrical structure is 0.5 mm to 1.5 mm, and the side length of the square opening position is 0.5 mm to 1.5 mm,   or,   the diameters of the end faces of the first end and the second end of the column structure are 1 mm to 6 mm;   the inner diameter of the hollow cylinder is 10 mm to 26 mm, and the side length of the square opening position is 10 mm to 26 mm,   wherein an input voltage signal of a piezoelectric transducer is 0.3 V to 0.8 V, a frequency of the input voltage signal is 25 kHz to 35 kHz, and a phase difference of vibration signals generated by two piezoelectric transducers is −10° to 10° or 80° to 100°.   
     
     
         15 - 16 . (canceled) 
     
     
         17 . The culture structure according to  claim 4 , wherein the vibration member is a cuboid structure, the accommodating structure is a hollow cuboid structure, and an orthographic projection of the accommodating structure on the cover plate covers an orthographic projection of the vibration member on the cover plate,
 wherein end faces of a first end and a second end of the cuboid structure are both square, and a side length of the square is 4 mm to 12 mm; and   an opening position of the accommodating structure is square, a side length of the square opening position is 14 mm to 22 mm, and a plane where the opening position is located is parallel to a plane where the cover plate is located.   
     
     
         18 . (canceled) 
     
     
         19 . The culture structure according to  claim 4 , wherein the vibration member is a column structure, end faces of a first end and a second end of the column structure are circular, any one of the accommodating structures is corresponding to two column structures, and orthographic projections of the two column structures on the cover plate are within a range of an orthographic projection of the accommodating structure corresponding to the two column structures on the cover plate,
 wherein diameters of the end faces of the first end and the second end of the column structure are 0.1 mm to 0.4 mm; and a spacing between two vibration members corresponding to a same accommodating structure is 0.1 mm to 0.3 mm; and   the accommodating structure is a hollow cylindrical structure, and an inner diameter of the hollow cylindrical structure is 1.1 mm to 3.2 mm; or the accommodating structure is a hollow cuboid structure, an opening position of the accommodating structure is a square, a side length of the square opening position is 1.1 mm to 3.2 mm, and a plane where the opening position is located is parallel to a plane where the cover plate is located.   
     
     
         20 . (canceled) 
     
     
         21 . The culture structure according to  claim 10 , wherein diameters of end faces of first ends and second ends of the plurality of vibration members gradually decrease from being close to a piezoelectric transducer to being far away from the piezoelectric transducer. 
     
     
         22 . The culture structure according to  claim 17 , wherein side lengths of end faces of first ends and second ends of the plurality of vibration members gradually decrease from being close to a piezoelectric transducer to being away from the piezoelectric transducer. 
     
     
         23 . The culture structure according to  claim 1 , wherein the vibration signal generating structure comprises a plurality of piezoelectric transducers, the vibration members are located at positions of a bottom of the culture plate corresponding to the plurality of accommodating structures respectively, the plurality of piezoelectric transducers are respectively corresponding to the plurality of vibration members, and the vibration signal generated by each piezoelectric transducer is applied to a corresponding vibration member,
 wherein the piezoelectric transducer is an annular piezoelectric transducer; and   an orthographic projection of the piezoelectric transducer on the cover plate is at least partially overlapped with an orthographic projection of the corresponding vibration member on the cover plate.   
     
     
         24 . (canceled) 
     
     
         25 . The culture structure according to  claim 10 , wherein an input voltage signal of a piezoelectric transducer is 0.3 V to 0.8 V, a frequency of the input voltage signal is 20 kHz to 40 kHz, and a phase difference of vibration signals generated by two piezoelectric transducers is 80° to 100°. 
     
     
         26 . (canceled) 
     
     
         27 . A culture method, adopting the culture structure according to  claim 1 , the culture structure comprising a culture plate and a vibration structure, the culture plate comprising a plurality of accommodating structures configured to accommodate culture solution; and the vibration structure comprising a vibration signal generating structure and a plurality of vibration members; wherein the method comprises:
 generating a vibration signal; and   driving, by the vibration structure, the culture solution in the accommodating structure to move according to the vibration signal.   
     
     
         28 . The culture method according to  claim 27 , wherein the vibration structure further comprises a cover plate, the accommodating structure comprises an opening arranged to face a side of the cover plate, the cover plate is provided on a side of the culture plate located at the opening of the accommodating structure, a plurality of vibration members are provided at a side of the cover plate facing the culture plate, any one of the vibration members is arranged corresponding to one of the accommodating structures; a vibration member comprises a first end and a second end which are arranged opposite to each other, the first end of the vibration member is connected to the cover plate, the second end of the vibration member extends away from the cover plate into the culture solution of a corresponding accommodating structure; and the vibration signal generating structure is provided on the cover plate;
 the generating the vibration signal comprises: applying a voltage signal to the vibration signal generating structure, and generating the vibration signal, by the vibration signal generating structure, according to the voltage signal; and   the driving, by the vibration structure, the culture solution in the accommodating structure to move according to the vibration signal, comprises: applying the vibration signal to the vibration member through the cover plate, and driving, by the vibration member, the culture solution in the corresponding accommodating structure to move.   
     
     
         29 . A culture chip comprising the culture structure according to  claim 1 .