High affinity pd-1 agents and methods of use

Abstract

High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

1 . A high affinity PD-1 mimic polypeptide comprising an amino acid sequence having 85% or more sequence identity to the amino acid sequence as set forth in SEQ ID NO: 2 and lacking a wild-type PD-1 transmembrane domain, wherein relative to the human wild-type PD-1 polypeptide as set forth in SEQ ID NO: 2, the high affinity PD-1 mimic polypeptide comprises one or more amino acid changes that increase the affinity of the high affinity PD-1 mimic polypeptide for PD-L1 as compared to the affinity for PD-L1 of the human wild type PD-1 polypeptide, the one or more amino acid changes located at an amino acid position selected from: V39, L40, N41, Y43, R44, M45, S48, N49, Q50, T51, D52, K53, A56, Q63, G65, Q66, V72, H82, M83, R90, Y96, L97, A 100, S102, L103, A104, P105, K106, and A107, and wherein the high affinity PD-1 mimic polypeptide comprises a fusion partner. 
     
     
         2 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide has a K d  of 1×10 −7  M or less for PD-L1. 
     
     
         3 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the one or more amino acid changes are located at an amino acid position selected from: V39, N41, Y43, M45, S48, N49, Q50, T51, D52, K53, A56, Q63, G65, Q66, L97, S102, L103, A104, P105, K106, and A107. 
     
     
         4 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the one or more amino acid changes are selected from: (1) V39H or V39R; (2) L40V or L40I; (3) N41I or N41V; (4) Y43F or Y43H; (5) R44Y or R44L; (6) M45Q, M45E, M45L, or M45D; (7) S48D, S48L, S48N, S48G, or S48V; (8) N49C, N49G, N49Y, or N49S; (9) Q50K, Q50E, or Q50H; (10) T51V, T51L, or T51A; (11) D52F, D52R, D52Y, or D52V; (12) K53T or K53L; (13) A56S or A56L; (14) Q63T, Q63I, Q63E, Q63L, or Q63P; (15) G65N, G65R, G65I, G65L, G65F, or G65V; (16) Q66P; (17) V72I; (18) H82Q; (19) M83L or M83F; (20) R90K; (21) Y96F; (22) L97Y, L97V, or L97I; (23) A100I or A100V; (24) S102T or S102A; (25) L103I, L103Y, or L103F; (26) A104S, A104H, or A104D; (27) P105A; (28) K106G, K106E, K106I, K106V, K106R, or K106T; and (29) A107P, A107I, or A107V. 
     
     
         5 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the one or more amino acid changes are amino acid changes located at amino acid positions selected from:
 (a) V39, N41, Y43, M45, S48, N49, Q50, K53, A56, Q63, G65, Q66, L97, S102, L103, A104, K106, and A107;   (b) V39, L40, N41, Y43, R44, M45, N49, K53, M83, L97, A100, and A107;   (c) V39, L40, N41, Y43, M45, N49, K53, Q66, M83, L97, and A107;   (d) V39, L40, N41, Y43, M45, N49, K53, Q66, H82, M83, L97, A100, and A107;   (e) V39, L40, N41, Y43, M45, N49, K53, M83, L97, A100, and A107;   (f) V39, L40, N41, Y43, R44, M45, N49, K53, L97, A100, and A107; and   (g) V39, L40, N41, Y43, M45, N49, K53, L97, A100, and A107.   
     
     
         6 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the one or more amino acid changes are amino acid changes selected from:
 (a) {V39H or V39R}, {N41I or N41V}, {Y43F or Y43H}, {M45Q, M45E, M45L, or M45D}, {S48D, S48L, S48N, S48G, or S48V}, {N49C, N49G, N49Y, or N49S}, {Q50K, Q50E, or Q50H}, {K53T or K53L}, {A56S or A56L}, {Q63T, Q63I, Q63E, Q63L, or Q63P}, {G65N, G65R, G65I, G65L, G65F, or G65V}, {Q66P}, {L97Y, L97V, or L97I}, {S102T or S102A}, {L103I, L103Y, or L103F}, {A104S, A104H, or A104D}, {K106G, K106E, K106I, K106V, K106R, or K106T}, and {A107P, A107I, or A107V};   (b) {V39H or V39R}, {L40V or L40I}, {N41I or N41V}, {Y43F or Y43H}, {R44Y or R44L}, {M45Q, M45E, M45L, or M45D}, {N49C, N49G, N49Y, or N49S}, {K53T or K53L}, {M83L or M83F}, {L97Y, L97V, or L97I}, {A100I or A100V}, and {A107P, A107I, or A107V};   (c) {V39H or V39R}, {L40V or L40I}, {N41I or N41V}, {Y43F or Y43H}, {M45Q, M45E, M45L, or M45D}, {N49C, N49G, N49Y, or N49S}, {K53T or K53L}, {Q66P}, {M83L or M83F}, {L97Y, L97V, or L97I}, and {A107P, A107I, or A107V};   (d) {V39H or V39R}, {L40V or L40I}, {N41I or N41V}, {Y43F or Y43H}, {M45Q, M45E, M45L, or M45D}, {N49C, N49G, N49Y, or N49S}, {K53T or K53L}, {Q66P}, {H82Q}, {M83L or M83F}, {L97Y, L97V, or L97I}, {A100I or A100V}, and {A107P, A107I, or A107V};   (e) {V39H or V39R}, {L40V or L40I}, {N41I or N41V}, {Y43F or Y43H}, {M45Q, M45E, M45L, or M45D}, {N49C, N49G, N49Y, or N49S}, {K53T or K53L}, {M83L or M83F}, {L97Y, L97V, or L97I}, {A100I or A100V}, and {A107P, A107I, or A107V};   (f) {V39H or V39R}, {L40V or L40I}, {N41I or N41V}, {Y43F or Y43H}, {R44Y or R44L}, {M45Q, M45E, M45L, or M45D}, {N49C, N49G, N49Y, or N49S}, {K53T or K53L}, {L97Y, L97V, or L97I}, {A100I or A100V}, and {A107P, A107I, or A 107V}; and   (g) {V39H or V39R}, {L40V or L40I}, {N41I or N41V}, {Y43F or Y43H}, {M45Q, M45E, M45L, or M45D}, {N49C, N49G, N49Y, or N49S}, {K53T or K53L}, {L97Y, L97V, or L97I}, {A100I or A100V}, and {A107P, A107I, or A107V}.   
     
     
         7 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the one or more amino acid changes are amino acid changes selected from:
 (a) V39R, N41V, Y43H, M45E, S48G, N49Y, Q50E, K53T, A56S, Q63T, G65L, Q66P, L97V, S102A, L103F, A104H, K106V, and A107I;   (b) V39H, L40V, N41V, Y43H, R44Y, M45E, N49G, K53T, M83L, L97V, A100I, and A107I;   (c) V39H, L40V, N41V, Y43H, M45E, N49G, K53T, Q66P, M83L, L97V, and A107I;   (d) V39H, L40V, N41V, Y43H, M45E, N49S, K53T, Q66P, H82Q, M83L, L97V, A100V, and A107I;   (e) V39H, L40I, N41I, Y43H, M45E, N49G, K53T, M83L, L97V, A100V, and A107I;   (f) V39H, L40V, N41I, Y43H, R44L, M45E, N49G, K53T, L97V, A100V, and A107I;   (g) V39H, L40V, N41I, Y43H, M45E, N49G, K53T, L97V, A100V, and A107I; and   (h) V39H, L40V, N41V, Y43H, M45E, N49G, K53T, L97V, A100V, and A107I.   
     
     
         8 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the one or more amino acid changes are amino acid changes selected from:
 (a) V39H, L40V, N41I, Y43H, M45E, N49G, K53T, L97V, A100V, and A107I; and   (b) V39H, L40V, N41V, Y43H, M45E, N49G, K53T, L97V, A100V, and A107I.   
     
     
         9 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide comprises the immunoglobulin domain of the amino acid sequence as set forth in any one of SEQ ID NOs: 3-13, 15-23 and 39-46. 
     
     
         10 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide comprises the amino acid sequence as set forth in any one of SEQ ID NOs: 3-13, 15-23 and 44-46. 
     
     
         11 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide comprises the immunoglobulin domain of the amino acid sequence as set forth in SEQ ID NO: 3. 
     
     
         12 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide comprises the amino acid sequence as set forth in SEQ ID NO: 3. 
     
     
         13 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide comprises the immunoglobulin domain of the amino acid sequence as set forth in SEQ ID NO: 4. 
     
     
         14 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the high affinity PD-1 mimic polypeptide comprises the amino acid sequence as set forth in SEQ ID NO: 4. 
     
     
         15 . The high affinity PD-1 mimic polypeptide of  claim 1 , wherein the fusion partner comprises a human immunoglobulin Fc region.