Method for treating alopecia with stem cell exosome

Abstract

A method for treating alopecia with a stem cell exosome, the alopecia includes androgenetic alopecia, seborrheic alopecia, and post-hair transplantation alopecia. An umbilical cord mesenchymal stem cell exosome has a significant curative effect on androgenetic alopecia, seborrheic alopecia, and post-hair transplantation alopecia.

1 . A method for treating alopecia with a stem cell exosome, wherein the alopecia comprises androgenetic alopecia, seborrheic alopecia, or post-hair transplantation alopecia. 
     
     
         2 . The method according to  claim 1 , wherein the stem cell exosome is one or more selected from the group consisting of a human placenta mesenchymal stem cell exosome, a human umbilical cord mesenchymal stem cell exosome, and a human amniotic fluid mesenchymal stem cell exosome. 
     
     
         3 . The method according to  claim 1 , wherein a preparation method of the stem cell exosome comprises:
 culturing mesenchymal stem cells for 24 h to 48 h and collecting a cell supernatant;   conducting centrifugation on the cell supernatant 2 to 4 times and filtering to obtain a filtrate; conducting centrifugation on the filtrate 2 to 4 times, discarding a first pellet, and conducting centrifugation to collect a second pellet; and   resuspending the second pellet with a PBS buffer to obtain a stem cell exosome solution.   
     
     
         4 . The method according to  claim 3 , wherein a medium of the mesenchymal stem cells comprises fetal bovine serum (FBS), Dulbecco's modified eagle medium (DMEM), and F12 at a ratio of (0.5-1.5):(3-6):(3-6). 
     
     
         5 . The method according to  claim 3 , wherein the centrifugation is conducted on the cell supernatant by:
 conducting centrifugation on the cell supernatant at 400 g to 600 g for 5 min to 15 min at 3° C. to 5° C. to obtain a first supernatant;   conducting centrifugation on the supernatant I at 1,500 g to 2,500 g for 10 min to 20 min at 3° C. to 5° C. to obtain a second supernatant; and   transferring the second supernatant and conducting centrifugation at 8,000 g to 12,000 g for 30 min at 3° C. to 5° C.   
     
     
         6 . The method according to  claim 3 , wherein the centrifugation is conducted to collect the second pellet II by:
 conducting centrifugation at 300 g to 500 g for 10 min to 20 min at 4° C. to 6° C. to obtain a first supernatant I;   conducting centrifugation on the supernatant I at 2,000 g to 3,000 g for 10 min to 20 min at 4° C. to 6° C. to obtain a second supernatant;   conducting centrifugation on the second supernatant at 10,000 g to 15,000 g for 25 min to 35 min at 4° C. to 6° C. to obtain a third supernatant; and   conducting centrifugation on the third supernatant at 125,000 g to 175,000 g for 50 min to 70 min at 4° C. to 6° C. to obtain the second pellet.   
     
     
         7 . The method according to  claim 1 , wherein the second pellet is resuspended with the PBS buffer by: resuspending the second pellet with the PBS buffer, conducting centrifugation at 125,000 g to 175,000 g for 50 min to 70 min at 4° C. to 6° C., discarding a supernatant to obtain a third pellet, and resuspending the third pellet in the PBS buffer. 
     
     
         8 . A stem cell exosome freeze-dried powder, prepared by a method comprising pre-freezing a stem cell exosome solution at −80° C. to −95° C., and vacuum freeze-drying at −40° C. to −60° C. to obtain the stem cell exosome freeze-dried powder, wherein the stem cell exosome is prepared by a method comprising:
 culturing mesenchymal stem cells for 24 h to 48 h and collecting a cell supernatant; conducting centrifugation on the cell supernatant 2 to 4 times and filtering to obtain a filtrate; 
 conducting centrifugation on the filtrate 2 to 4 times, discarding a first pellet, and conducting centrifugation to collect a second pellet; and 
 resuspending the second pellet with a PBS buffer to obtain a stem cell exosome solution. 
 
     
     
         9 . A stem cell exosome-based thermosensitive gel, comprising the following components by weight percentage: 0.5% to 1.5% of the stem cell exosome freeze-dried powder according to  claim 8 , 10% to 30% of poloxamer 407, and 2% to 6% of poloxamer 188.