Cell Culture Plate, Assembly And Methods Of Use

Abstract

A cell culture system or assembly, flask, plate and dish for growing, viewing and evaluating cells; and methods of use.

What is claimed is: 
     
         1 . A cell culture assembly for holding one or more cells, the assembly comprising:
 a slide comprising:
 a top surface; 
 a bottom surface; 
 at least one edge; and 
 at least one projection attached to said edge; and 
   a cover slip comprising:
 a top surface; 
 a bottom surface; and 
 at least one aperture to accommodate said at least one projection; 
   
       wherein said cover slip overlaps with at least a portion of said slide; and 
       wherein said cover slip is transparent. 
     
     
         2 . The cell culture assembly of  claim 1 , wherein said cover slip further comprises an adhesive applied on said bottom surface capable of bonding said top surface of said slide to said cover slip. 
     
     
         3 . The cell culture assembly of  claim 1 , wherein said cover slip further comprises a labeling region. 
     
     
         4 . The cell culture assembly of  claim 1 , wherein said cover slip is transparent to visible light. 
     
     
         5 . The cell culture assembly of  claim 1 , wherein said slide is sterile. 
     
     
         6 . The cell culture assembly of  claim 1 , wherein said at least one projection is self-locking in said aperture. 
     
     
         7 . A cell culture assembly for holding one or more cells, the assembly comprising:
 a slide comprising:
 a top surface; 
 a bottom surface; and 
 at least one edge; 
   a cover slip comprising:
 a top surface; 
 a bottom surface; and 
 at least one edge; 
   wherein said cover slip is transparent; and   a hinge connecting said slide and said cover slip.   
     
     
         8 . The cell culture assembly of  claim 7 , wherein said cover slip further comprises an adhesive applied on said bottom surface capable of bonding said top surface of said slide to said cover slip. 
     
     
         9 . The cell culture assembly of  claim 7 , wherein said cover slip further comprises a labeling region. 
     
     
         10 . The cell culture assembly of  claim 7 , wherein said cover slip is transparent to visible light. 
     
     
         11 . The cell culture assembly of  claim 7 , wherein said slide is sterile. 
     
     
         12 .- 17 . (canceled) 
     
     
         18 . A cell culture plate for culturing one or more cells, the cell culture plate comprising:
 a spherical container comprising:
 an internal cavity; and 
 a vent; and 
 a lid comprising: 
 a top surface, 
   wherein the top surface of the lid comprises a grip portion.   
     
     
         19 . The cell culture plate of  claim 18 , wherein the vent comprises a liquid permeable gas permeable membrane. 
     
     
         20 . The cell culture plate of  claim 19 , wherein the liquid permeable gas permeable membrane comprises a microporous membrane. 
     
     
         21 . The cell culture plate of  claim 18 , further comprising a slide, wherein the slide comprises:
 a top surface;   a bottom surface;   at least one edge; and   at least one projection attached to said edge.   
     
     
         22 .- 39 . (canceled)