Detergent compositions and uses thereof

Abstract

The present invention relates to compositions such as cleaning compositions comprising a mix of enzymes. The invention further relates, use of compositions comprising such enzymes in cleaning processes.

1 . A cleaning composition comprising at least 0.01 ppm DNase, at least 0.01 ppm hexosaminidase and cleaning component(s), wherein the cleaning component(s) is selected from a group consisting of:
 a. 0.1 to 60 wt % of at least one surfactant;   b. 0 to 50 wt % of a builder; and   c. 0 to 20 wt % of a bleach component, and   d. combinations thereof.   
     
     
         2 . The cleaning composition of  claim 1 , wherein the DNase is a NUC1 or NUC1A DNase and comprises one or both of the motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO: 73), ASXNRSKG (SEQ ID NO: 74). 
     
     
         3 . The cleaning composition of  claim 1 , wherein the DNase is selected from the group consisting of:
 a) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1,   b) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2,   c) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3,   d) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4,   e) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5,   f) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6,   g) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7,   h) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8,   i) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9,   j) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10,   k) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11,   l) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12,   m) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13,   n) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 14,   o) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15,   p) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16,   q) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17,   r) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18,   s) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19,   t) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20,   u) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21,   v) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22,   w) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23,   x) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, and   y) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25, and   combinations thereof.   
     
     
         4 . The cleaning composition of  claim 1 , wherein the DNase is a NUC1 or NUC1A DNase and comprises one or both of the motifs [V/I]PL[S/A]NAWK (SEQ ID NO: 75) or NPQL (SEQ ID NO: 76). 
     
     
         5 . The cleaning composition of  claim 1 , wherein the DNase is selected from the group consisting of:
 a) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26,   b) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27,   c) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28,   d) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29,   e) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30,   f) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31,   g) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32,   h) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33,   i) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34,   j) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35,   k) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36,   l) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, and   m) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38, and   combinations thereof.   
     
     
         6 . The cleaning composition of  claim 1 , wherein the DNase is a NUC1 or NUC1A DNase and comprises one or both of the motifs P[Q/E]L[W/Y] or [K/H/E]NAW. 
     
     
         7 . The cleaning composition of  claim 1 , wherein the DNase is selected from the group consisting of:
 a) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39,   b) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40,   c) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41,   d) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42,   e) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43   f) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44,   g) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45,   h) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46,   i) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47,   j) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48,   k) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49,   l) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, and   m) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51, and   combinations thereof.   
     
     
         8 . The cleaning composition of  claim 1 , wherein the DNase is selected from the group consisting of:
 a) polypeptide obtainable from  Bacillus licheniformis  having a sequence identity to the polypeptide shown in SEQ ID NO: 65 of at least 60% and which have DNase activity,   b) polypeptide obtainable from  Bacillus subtilis  having a sequence identity to the polypeptide shown in SEQ ID NO: 66 of at least 60% and which have DNase activity,   c) polypeptide obtainable from  Aspergillus oryzae  having a sequence identity to the polypeptide shown in SEQ ID NO: 67 of at least 60% and which have DNase activity,   d) polypeptide obtainable from  Trichoderma harzianum  having a sequence identity to the polypeptide shown in SEQ ID NO: 68 of at least 60% and which have DNase activity, and   combinations thereof.   
     
     
         9 . The cleaning composition of  claim 1 , wherein the hexosaminidase is selected from the group consisting of:
 a) a polypeptide having hexosaminidase activity selected from the group consisting of: a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 82, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 83, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 84, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 85, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 86, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 87, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 88, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 89, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 90, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 91, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 92, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 93; a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 94; a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 95; a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 96; a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 97; a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 98; a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 99;   b) a polypeptide having N-acetylglucosaminidase activity, preferably β-N-acetylglucosaminidase activity; and   c) a polypeptide comprising a GH20 domain, and   d) combinations thereof.   
     
     
         10 - 11 . (canceled) 
     
     
         12 . A kit intended for deep cleaning, wherein the kit comprises a solution of an enzyme mixture comprising a DNase, and a hexosaminidase. 
     
     
         13 . A method of deep cleaning of an item, comprising the steps of:
 a) contacting the item with a wash liquor solution comprising an enzyme mixture comprising at least 0.00001 ppm of a DNase, at least 0.00001 ppm of a hexosaminidase; and a cleaning component, wherein the cleaning component comprises at least a surfactant; optionally a builder, and optionally a bleach component; and   b) optionally rinsing the item,   
       wherein the item is a textile.