Modified mon89788 soybean transgenic herbicide tolerance locus

Abstract

Transgenic INHT31 soybean plants comprising modifications of the MON89788 soybean locus which provide for facile excision of the modified MON89788 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

1 . A transgenic soybean plant cell comprising a modification of a MON89788 transgenic soybean locus, wherein the modification comprises:
 (i) a deletion of DNA in a 5′ or a 3′ DNA junction polynucleotide of a MON89788 transgenic locus; and/or   (ii) an insertion and/or substitution of DNA in a DNA junction polynucleotide of a MON89788 transgenic locus with DNA comprising a cognate guide RNA recognition site (CgRRS).   
     
     
         2 . The transgenic soybean plant cell of  claim 1 , wherein the CgRRS is inserted in the 3′ DNA junction polynucleotide of a MON89788 transgenic locus and wherein the modifications further comprise a deletion of DNA in the 5′ DNA junction polynucleotide of the MON89788 transgenic locus. 
     
     
         3 . The transgenic soybean plant cell of  claim 1 , wherein the deletion of DNA in the 5′ DNA junction polynucleotide of the MON89788 transgenic locus comprise a deletion of one or more residues corresponding to nucleotides 1 to 204 of SEQ ID NO: 2 or wherein the deletion of DNA in of the 3′ junction polynucleotide comprise a deletion wherein one or more nucleotides in the 3′ junction polynucleotide corresponding to nucleotides 5397 to 5416 of SEQ ID NO: 1 are absent. 
     
     
         4 . The transgenic soybean plant cell of  claim 1 , wherein the deletion of DNA in the 5′ junction polynucleotide of the MON89788 transgenic locus comprise a deletion of DNA in the 5′ junction polynucleotide wherein one or more nucleotides in a segment corresponding to nucleotides 1094 to 1113 of SEQ ID NO: 1 are absent. 
     
     
         5 . The transgenic soybean plant cell of  claim 1 , wherein the CgRRS is inserted in the 3′ junction polynucleotide of a MON89788 transgenic locus and comprises the DNA molecule set forth in SEQ ID NO: 16 or 17. 
     
     
         6 . The transgenic soybean plant cell of  claim 1 , wherein the CgRRS is inserted into the 5′ junction polynucleotide of the MON89788 and/or wherein one or more nucleotides in the 3′ junction polynucleotide corresponding to nucleotides 5397 to 5416 of SEQ ID NO: 1 are absent. 
     
     
         7 . A transgenic soybean plant part comprising the transgenic soybean cell of  claim 1  wherein the soybean plant part comprises the modification of a MON89788 transgenic soybean locus. 
     
     
         8 . The transgenic soybean plant part of  claim 7 , wherein the plant part is a seed. 
     
     
         9 . A transgenic soybean plant comprising the transgenic soybean cell of  claim 1  wherein the soybean plant comprises the modification of the MON89788 transgenic locus. 
     
     
         10 . A method for obtaining a bulked population of inbred seed comprising selfing the transgenic soybean plant of  claim 9  and harvesting seed comprising the modification of a MON89788 transgenic locus from the selfed soybean plant. 
     
     
         11 . A method of obtaining hybrid soybean seed comprising crossing the transgenic soybean plant of  claim 9  to a second soybean plant which is genetically distinct from the first soybean plant and harvesting seed comprising the modification of the MON89788 transgenic locus from the cross. 
     
     
         12 . A processed transgenic soybean plant product comprising the DNA molecule comprising an insertion and/or substitution of DNA in a DNA junction polynucleotide of a MON89788 transgenic locus with DNA comprising a cognate guide RNA recognition site (CgRRS).