Organ mimic device with microchannels and methods of use and manufacturing thereof

Abstract

System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

1 - 48 . (canceled) 
     
     
         49 . A microfluidic device comprising first and second microchannels, wherein said microchannels are fluidically connected by a membrane positioned between said microchannels, said membrane comprising first and second surfaces, said first surface comprising a layer of epithelial cells and said second surface comprising a layer of endothelial cells, wherein said membrane comprises pores large enough to permit migration of epithelial cells and endothelial cells, and wherein the cells on said first and/or second surface are polarized. 
     
     
         50 . The microfluidic device of  claim 49 , wherein said epithelial cells are lung cells. 
     
     
         51 . The microfluidic device of  claim 49 , wherein said epithelial cells are kidney cells.