Methods and compositions for partitioning a biological sample

Abstract

This disclosure relates to compositions and methods for analyzing a tissue section from a biological sample.

What is claimed is: 
     
         1 . A composition comprising:
 (a) a biological sample embedded in a polymer solution;   (b) a non-aqueous droplet, wherein the non-aqueous droplet surrounds the biological sample;   (c) one or more reagents to manipulate the biological sample, wherein the one or more reagents are selected from the group consisting of:
 (A) a plurality of capture probes, wherein a capture probe of the plurality comprises (i) a spatial barcode and (ii) a capture domain that binds to a sequence present in an analyte; 
 (B) a plurality of analyte capture agents, wherein an analyte capture agent of the plurality of analyte capture agents comprises:
 an analyte binding moiety that binds to the analyte; 
 an analyte binding moiety barcode that uniquely identifies an interaction between the analyte and the analyte binding moiety; and 
 an analyte capture sequence, wherein the analyte capture sequence binds to a capture domain; and 
 
 (C) a protein-binding molecule for immunofluorescence or immunohistochemistry; and 
   (d) a substrate comprising a plurality of probes that is capable of detecting the analyte, the capture probe, or the analyte capture agent.