Hybrid biological membranes, methods of making and uses thereof

Abstract

Described herein is a hybrid biological membrane comprising an endogenous bilayer doped with one or more synthetic lipid molecules. Also described herein is a method of preparing a hybrid biological membrane, the method comprising doping an endogenous bilayer with one or more synthetic lipid molecules.

What is claimed is: 
     
         1 . A hybrid biological membrane comprising an endogenous bilayer doped with one or more modifying lipid molecules, wherein the endogenous bilayer lipids and the modifying lipid molecules anneal to form a substantially homogenous hybrid biological membrane structure. 
     
     
         2 . The membrane of  claim 1 , wherein the one or more modifying lipid molecules are fully saturated, partially saturated, or fully unsaturated. 
     
     
         3 . The membrane of  claim 1 , wherein the modifying lipids are endogenous or non-endogenous. 
     
     
         4 . The membrane of  claim 1 , wherein the modifying lipids are natural or non-natural. 
     
     
         5 . The membrane of  claim 1 , wherein the modifying lipids are synthetically produced. 
     
     
         6 . The membrane of  claims 1 , wherein the one or more modifying lipid molecules comprise phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidic acid, dimyristoylphosphatidylcholine, palmitoyloleoylglycerophosphocholine, palmitoyloleoylglycerophosphoserine, palmitoyloleoylglycerophosphoglycerol, sphingomyelin, phosphoethanolamine, a variant thereof, a derivative thereof, or a combination thereof. 
     
     
         7 . The membrane of  claim 1 , wherein the ratio of endogenous bilayer lipids:modifying lipids is from about 1:10 to about 20:1. 
     
     
         8 . The membrane of  claim 1 , wherein the modifying lipid molecules functionalize the biological membrane by altering its thickness, order, and/or surface charge. 
     
     
         9 . The membrane of  claim 1 , wherein the endogenous bilayer is an erythrocyte bilayer. 
     
     
         10 . The membrane of  claim 1 , comprising from about 5 mass % to about 50 mass % modifying lipid molecules. 
     
     
         11 . The membrane of  claim 1 , wherein the membrane is biocompatible. 
     
     
         12 . The membrane of  claim 1 , wherein the membrane is resistant to mechanical and/or osmotic stress. 
     
     
         13 . The membrane of  claim 1 , wherein the membrane further comprises one or more biomolecules and/or small molecules. 
     
     
         14 . The membrane of  claim 1 , wherein the membrane encapsulates a releasable cargo comprising a biomolecule or a small molecule, wherein the releasable cargo comprises a therapeutic agent, a prophylactic agent, a diagnostic agent, a marker agent, a prognostic agent, or a combination thereof. 
     
     
         15 . The membrane of  claim 14 , wherein the releasable cargo comprises an antibiotic. 
     
     
         16 . A hybrid biological membrane comprising an endogenous bilayer doped with one or more anionic modifying lipid molecules. 
     
     
         17 . The membrane of  claim 16 , wherein the one or more anionic modifying lipid molecules comprise palmitoyloleoylglycerophosphoserine (POPS), palmitoyloleoylglycerophospho-glycerol) (POPG), or a combination thereof.