ANTIBODY COMPOSITIONS TARGETING NON-PHOSPHORYLATED a-SYNUCLEIN AGGREGATES

Abstract

The present specification provides a monoclonal antibody that specifically binds aggregated, non-phosphorylated α-synuclein and a hybridoma producing it. Also disclosed are methods of generating antibodies that specifically binds aggregated, non-phosphorylated α-synuclein and uses thereof. Uses of anti-α-synuclein antibody in detection and diagnostic assays, and for prophylaxis or therapy of α-synuclein-associated neurodegenerative diseases, are also disclosed.

1 . A hybridoma cell line as deposited with the American Type Culture Collection under patent deposit accession number PTA-127017. 
     
     
         2 . A monoclonal antibody produced by the hybridoma of  claim 1 . 
     
     
         3 . A hybridoma generated by a method comprising immunizing a mouse with a peptide comprising amino acid residues 125-133 of α-synuclein (SEQ ID NO: 1). 
     
     
         4 . The hybridoma of  claim 3 , wherein the peptide comprising amino acid residues 125-133 of α-synuclein is CYEMPSEEGY (SEQ ID NO: 3). 
     
     
         5 . A monoclonal antibody produced by the hybridoma of  claim 2 . 
     
     
         6 . A monoclonal antibody made by a process comprising immunizing a laboratory animal with means for inducing antibodies recognizing aggregated, non-phosphorylated α-synuclein. 
     
     
         7 . The monoclonal antibody of  claim 6 , wherein the laboratory animal is a mouse. 
     
     
         8 . The monoclonal antibody of  claim 6 , wherein the means for inducing antibodies recognizing aggregated, non-phosphorylated α-synuclein are conjugated to a carrier protein. 
     
     
         9 . The monoclonal antibody of  claim 8 , wherein the carrier protein is keyhole limpet hemocyanin. 
     
     
         10 . The monoclonal antibody of  claim 6 , wherein the means for inducing antibodies recognizing aggregated, non-phosphorylated α-synuclein is the peptide CYEMPSEEGY (SEQ ID NO:3). 
     
     
         11 . The monoclonal antibody of  claim 2 , that is a fragment of a whole antibody. 
     
     
         12 . An immunoassay for the detection or quantitation of aggregated, non-phosphorylated α-synuclein comprising a step for contacting a sample with means for binding WT-α-syn. 
     
     
         13 . The immunoassay of  claim 12 , wherein the means for binding WT-α-syn is the monoclonal antibody produced by the hybridoma deposited with the American Type Culture Collection under patent deposit accession number PTA-127017.