Method for preparing a self-assembled porous three-dimensional zinc oxide nanoparticle structure using a microfluidic device, zinc oxide nanoparticles, an aggregate of zinc oxide nanoparticles, and zinc oxide nanoparticle structure prepared therefrom, and a bio-ink including the same

Abstract

Provided is a method for preparing a zinc oxide nanoparticle structure synthesized using a microfluidic device and a self-assembled porous three-dimensional zinc oxide nanoparticle structure prepared therefrom. The self-assembled porous three-dimensional zinc oxide nanoparticle structure of the present application is a three-dimensional structure in which micropores, mesopores and macropores are created, and has excellent reactivity.

1 . A self-assembled porous three-dimensional zinc oxide nanoparticle structure comprising:
 monodisperse porous zinc oxide nanoparticles comprising channel-type micropores having a diameter of 0.1 nm to 2 nm in the nanoparticles and having a content of the zinc precursor dispersed in an amine compound at 1.67% (w/v) or more and 3.57% (w/v) or less; and   a porous aggregate of zinc oxide nanoparticles formed by aggregating the nanoparticles, wherein a distance between the nanoparticles comprises mesopores of 2 nm to 50 nm.   
     
     
         2 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the nanoparticle structure is prepared by a method comprising:
 generating a dispersion by mixing the zinc precursor and the amine compound;   generating the nanoparticles by applying heat of a first temperature while introducing the dispersion into a microfluidic channel in a microfluidic device;   aggregating nanoparticles by reacting a solution discharged from the microfluidic channel at a second temperature that is higher than the first temperature; and   after mixing the solution and a C1-C4 alcohol, drying the precipitate to obtain the nanoparticles or aggregates thereof.   
     
     
         3 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the distance between the nanoparticles further comprises macropores of 50 nm to 2.5 μm and the nanoparticle aggregate has a hierarchical network structure having a honeycomb shape. 
     
     
         4 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the nanoparticles have a particle size of 100 nm or less. 
     
     
         5 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the nanoparticle exhibits a positive zeta potential value. 
     
     
         6 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the nanoparticles have a mesopore volume of 0.1 cm3/g to 0.2 cm3/g, and a surface area of 20 cm2/g to 30 m2/g. 
     
     
         7 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the nanoparticles exhibit a maximum absorbance at a wavelength of 190 nm to 400 nm, and exhibits fluorescence when an excitation wavelength is 358 nm and an emission wavelength is 400 nm to 700 nm. 
     
     
         8 . The self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 , wherein the nanoparticles exhibit peaks of E2Low, A1Additional, A1TO, E2High, A1LO, E1LO, A1LO+E2High, A12LO+E12LO and A1TO+A1LO+E1High+E2High in a Raman spectrum. 
     
     
         9 . The self-assembled porous three-dimensional zinc oxide nanoparticles according to  claim 1 , wherein the nanoparticles exhibit binding energy peaks of oxygen (O) at 530 eV and zinc (Zn) at 1,030 eV, 8,640 eV, and 9,570 eV in an energy dispersive spectroscopy spectrum. 
     
     
         10 . A bio-ink comprising the self-assembled porous three-dimensional zinc oxide nanoparticle structure according to  claim 1 . 
     
     
         11 . A self-assembled porous aggregate of zinc oxide nanoparticles, wherein:
 each of the zinc oxide nanoparticles is a monodisperse spherical nanoparticle having a particle size of 100 nm or less and comprises channel-type micropores having a diameter of 0.1 nm to 2 nm in the nanoparticles; and   a distance between the nanoparticles comprises mesopores of 2 nm to 50 nm.