Selection based on optimal haploid value to create elite lines

Abstract

This disclosure concerns methods for estimating the breeding value of plants for the purpose of producing doubled haploid, for example, to identify selection candidates having high breeding values.

What may be claimed is: 
     
         1 . A method for producing an elite double haploid plant, the method comprising:
 crossing a first parent plant and a second parent plant to produce a population of F 1  plants;   producing a population of doubled haploids from at least one of the F 1  plants; and   selecting an elite doubled haploid plant from the population, wherein the selected double haploid has a combined haploid value (CHV) that approaches the highest possible optimal haploid value (OHV) for segregating individuals that can be produced from the breeding cross.   
     
     
         2 . The method of  claim 1 , wherein the selected plant has a CHV greater than the mean CHV of the population of doubled haploids. 
     
     
         3 . The method of  claim 1 , wherein the first parent plant comprises an allele of interest, wherein the second parent plant does not comprise the allele of interest, and wherein the selected plant also comprises the allele of interest. 
     
     
         4 . The method of  claim 3 , wherein the allele of interest is a transgenic event. 
     
     
         5 . A method for producing an elite double haploid plant, the method comprising:
 providing a first diploid parent plant and a plurality of candidate second diploid parental plants;   predicting genotypes that can be produced from crosses between the first parent plant and each of the candidate parental plants;   determining the optimal haploid value (OHV) for each of the predicted genotypes;   selecting the candidate second parental plant that is predicted in a cross with the first parent plant to produce the genotype having the highest OHV;   crossing the first parent plant with the selected second parental plant to produce an F 1  plant;   producing haploid plants or tissues from the F 1  plant;   determining the combined haploid value (CHV) for each of the haploid plants or tissues;   selecting at least one haploid plant or tissue having a CHV that approaches the OHV of the F 1  plant; and   producing a population of doubled haploids from each of the selected haploid plants or tissues.   
     
     
         6 . A method for introducing a desired trait into an elite plant variety, the method comprising:
 (a) crossing a first parent plant of an elite plant variety with a second parent plant of a different variety that comprises a desired trait to produce an F 1  plant;   (b) determining the optimal haploid value (OHV) for the F 1  plant;   (c) producing haploids from the F 1  plant;   (d) selecting a haploid that has a combined haploid value (CHV) that approaches the optimal haploid value (OHV), wherein the CHV is the sum of the haploid values for each of a plurality of genome segments in the plant, and the desired trait;   (e) producing a population of doubled haploids from the selected haploid; and   (f) performing steps (a) through (e) one or more times in succession, to produce the selected or higher backcross progeny plant that comprises the desired trait.   
     
     
         7 . The method of  claim 1 , wherein the plants are alfalfa, apple, banana, bean, broccoli, castorbean, citrus, clover, coconut, coffee, cucumber, Douglas fir,  Eucalyptus , Loblolly pine, linseed, melon, oat, olive, palm, pea, peanut, pepper, poplar,  Radiata  pine, sorghum, Southern pine, strawberry, sugarbeet, sugarcane, sunflower, sweetgum, tea, tobacco, tomato, turf,  Arabidopsis thaliana , barley, maize, cotton, rapeseed/canola, rice, rye, soybean, or wheat plants. 
     
     
         8 . The method of  claim 8 , wherein the plants are wheat plants. 
     
     
         9 . The method of  claim 8 , wherein the plants are maize plants. 
     
     
         10 . The method of  claim 5 , wherein the plants are alfalfa, apple, banana, bean, broccoli, castorbean, citrus, clover, coconut, coffee, cucumber, Douglas fir,  Eucalyptus , Loblolly pine, linseed, melon, oat, olive, palm, pea, peanut, pepper, poplar,  Radiata  pine, sorghum, Southern pine, strawberry, sugarbeet, sugarcane, sunflower, sweetgum, tea, tobacco, tomato, turf,  Arabidopsis thaliana , barley, maize, cotton, rapeseed/canola, rice, rye, soybean, or wheat plants. 
     
     
         11 . The method of  claim 10 , wherein the plants are wheat plants. 
     
     
         12 . The method of  claim 10 , wherein the plants are maize plants. 
     
     
         13 . The method of  claim 6 , wherein the plants are alfalfa, apple, banana, bean, broccoli, castorbean, citrus, clover, coconut, coffee, cucumber, Douglas fir,  Eucalyptus , Loblolly pine, linseed, melon, oat, olive, palm, pea, peanut, pepper, poplar,  Radiata  pine, sorghum, Southern pine, strawberry, sugarbeet, sugarcane, sunflower, sweetgum, tea, tobacco, tomato, turf,  Arabidopsis thaliana , barley, maize, cotton, rapeseed/canola, rice, rye, soybean, or wheat plants. 
     
     
         14 . The method of  claim 6 , wherein the plants are wheat plants. 
     
     
         15 . The method of  claim 6 , wherein the plants are maize plants.